{"created":"2023-05-15T14:35:04.552375+00:00","id":45279,"links":{},"metadata":{"_buckets":{"deposit":"7292ddc8-ff67-40c7-a57c-9c85ee9492a7"},"_deposit":{"created_by":1,"id":"45279","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45279"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045279","sets":["1"]},"author_link":["449807","449805","449809","449811","449804","449803","449802","449810","449812","449808","449806"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"540","bibliographicPageStart":"533","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We describe a novel method of rapid and quantitative evaluation of the degree of radiation-induced apoptosis in the developing brain of medaka (Oryzias latipes). Embryos at stage 28 were irradiated with 1, 2, 3.5, and 5 Gy of X-rays. Twenty to twenty-four hours after the irradiation, living embryos were stained with a vital dye, acridine orange (AO), for 1-2 h, and whole-mount brains were examined under an epifluorescence microscope. The numbers of AO-stained rosette-shaped nuclear clusters and AO-stained single nuclei increased in a dose-dependent manner in the optic tectum. Electron microscopy revealed that the AO-stained rosette-shaped nuclear clusters were the nuclei of aggregated apoptotic cells. We used the number of AO-stained rosette-shaped nuclear clusters/tectum as an index of the degree of radiation-induced brain cell death. The results showed that the number of rosette-shaped nuclear clusters/optic tectum in irradiated embryos exposed to higher than 2 Gy was highly significantly different from that in nonirradiated control embryos, whereas no difference was detected at 1 Gy. Thus, the threshold dose for brain cell death in medaka embryos is about 1 Gy and this dose is probably comparable to those for rodents and humans. The results show that medaka embryos are useful for quantitative evaluation of developmental neurocytotoxic effects of radiation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yasuda, Takako"}],"nameIdentifiers":[{"nameIdentifier":"449802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshimoto, Masami"}],"nameIdentifiers":[{"nameIdentifier":"449803","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maeda, Keiko"}],"nameIdentifiers":[{"nameIdentifier":"449804","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Atsuko"}],"nameIdentifiers":[{"nameIdentifier":"449805","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maruyama, Kouichi"}],"nameIdentifiers":[{"nameIdentifier":"449806","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuuji"}],"nameIdentifiers":[{"nameIdentifier":"449807","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"保田 隆子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449808","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"前田 圭子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449809","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 厚子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449810","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丸山 耕一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449811","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 裕二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449812","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Rapid and simple method for quantitative evaluation of neurocyototoxic effects of radiation on developing medaka brain","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rapid and simple method for quantitative evaluation of neurocyototoxic effects of radiation on developing medaka brain"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-02"},"publish_date":"2008-10-02","publish_status":"0","recid":"45279","relation_version_is_last":true,"title":["Rapid and simple method for quantitative evaluation of neurocyototoxic effects of radiation on developing medaka brain"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:05:37.262541+00:00"}