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Radiation-induced gamma-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam
https://repo.qst.go.jp/records/45241
https://repo.qst.go.jp/records/4524172f47008-827e-4ea4-ab2e-04d99b51d074
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-08-13 | |||||
タイトル | ||||||
タイトル | Radiation-induced gamma-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Usami, Noriko
× Usami, Noriko× Eguchi-Kasai, Kiyomi× Maezawa, Hiroshi× Kobayashi, Katsumi× et.al× 宇佐美 徳子× 笠井 清美× 前澤 博× 小林 克己 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In order to study the radiobiological effects from low dose radiation, a cell irradiation system using synchrotron X-ray microbeam has been developed, by which cells can be recognised individually and irradiated one by one with the desired dose of monochromatic X rays. The minimum beam sizes obtained are 2 um with the focusing optics and 5 um square with the non-focused beam, and the beam size can be changed easily with a high-precision slit in the case of a non-focused beam. Human fibroblast cells were individually irradiated with this system, and immunostained by gamma-H2AX antibody to visualise the DNA damage. Most of the fluorescent foci were observed in a localised area in cell nuclei, the size of which was almost the same as the beam size. | |||||
書誌情報 |
Radiation Protection Dosimetry 巻 122, 号 1/4, p. 307-309, 発行日 2006-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0144-8420 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/rpd/ncl434 |