@article{oai:repo.qst.go.jp:00045241, author = {Usami, Noriko and Eguchi-Kasai, Kiyomi and Maezawa, Hiroshi and Kobayashi, Katsumi and et.al and 宇佐美 徳子 and 笠井 清美 and 前澤 博 and 小林 克己}, issue = {1/4}, journal = {Radiation Protection Dosimetry}, month = {Dec}, note = {In order to study the radiobiological effects from low dose radiation, a cell irradiation system using synchrotron X-ray microbeam has been developed, by which cells can be recognised individually and irradiated one by one with the desired dose of monochromatic X rays. The minimum beam sizes obtained are 2 um with the focusing optics and 5 um square with the non-focused beam, and the beam size can be changed easily with a high-precision slit in the case of a non-focused beam. Human fibroblast cells were individually irradiated with this system, and immunostained by gamma-H2AX antibody to visualise the DNA damage. Most of the fluorescent foci were observed in a localised area in cell nuclei, the size of which was almost the same as the beam size.}, pages = {307--309}, title = {Radiation-induced gamma-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam}, volume = {122}, year = {2006} }