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Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo
https://repo.qst.go.jp/records/45140
https://repo.qst.go.jp/records/4514090dee410-9bbd-471a-b154-f9abd4d8a054
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-21 | |||||
| タイトル | ||||||
| タイトル | Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yu, Dong
× Yu, Dong× Sekine, Emiko× Fujimori, Akira× Ochiya, Takahiro× Okayasu, Ryuichi× 于 冬× 関根 絵美子× 藤森 亮× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to study the role of BRCA2 protein in homologous recombination (HR) repair and radio-sensitization, we utilized RNA interference (RNAi) strategy in vitro and in vivo with human tumor cells. HeLa cells transfected with BRCA2 siRNA (Qiagen) as well as negative-control siRNA for 48 hours were irradiated, and several critical end points were examined. The radiation cell survival level was significantly reduced in HeLa cells with BRCA2 siRNA when compared with mock- or negative control siRNA transfected cells. DNA double strand break (DSB) repair as measured by constant field gel-electrophoresis showed a clear inhibition in cells with BRCA2 siRNA, while little inhibition was observed in cells with negative control siRNA. Our immuno-staining experiments revealed a significant delay in Rad51 foci formation in cells with BRCA2 siRNA when compared with the control populations. However, none of the NHEJ proteins nor the phosphorylation of DNA-PKcs was affected in cells transfected with BRCA2 siRNA. In addition, the combined treatment with radiation and BRCA2 siRNA in xenograft model with HeLa cells showed an efficient inhibition of in vivo tumor growth. Our results demonstrate down-regulation of BRCA2 leads to radio-sensitization mainly through the inhibition of HRR type DSB repair; a possibility of using BRCA2 siRNA as an effective radiosensitizer in tumor radiotherapy may arise. |
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| 書誌情報 |
Cancer Science 巻 99, 号 4, p. 810-815, 発行日 2008-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1347-9032 | |||||
