{"created":"2023-05-15T14:34:55.379543+00:00","id":45140,"links":{},"metadata":{"_buckets":{"deposit":"e1f57999-12a6-495b-853f-dd1995cd6a97"},"_deposit":{"created_by":1,"id":"45140","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45140"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045140","sets":["1"]},"author_link":["448239","448235","448238","448240","448236","448234","448237","448242","448241"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"815","bibliographicPageStart":"810","bibliographicVolumeNumber":"99","bibliographic_titles":[{"bibliographic_title":"Cancer Science"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to study the role of BRCA2 protein in homologous recombination\n(HR) repair and radio-sensitization, we utilized RNA interference (RNAi)\nstrategy in vitro and in vivo with human tumor cells. HeLa cells\ntransfected with BRCA2 siRNA (Qiagen) as well as negative-control siRNA\nfor 48 hours were irradiated, and several critical end points were\nexamined. The radiation cell survival level was significantly reduced in\nHeLa cells with BRCA2 siRNA when compared with mock- or negative control\nsiRNA transfected cells.  DNA double strand break (DSB) repair as\nmeasured by constant field gel-electrophoresis showed a clear inhibition\nin cells with BRCA2 siRNA, while little inhibition was observed in cells\nwith negative control siRNA. Our immuno-staining experiments revealed a\nsignificant delay in Rad51 foci formation in cells with BRCA2 siRNA when\ncompared with the control populations. However, none of the NHEJ\nproteins nor the phosphorylation of DNA-PKcs was affected in cells\ntransfected with BRCA2 siRNA. In addition, the combined treatment with\nradiation and BRCA2 siRNA in xenograft model with HeLa cells showed an\nefficient inhibition of in vivo tumor growth. Our results demonstrate\ndown-regulation of BRCA2 leads to radio-sensitization mainly through the\ninhibition of HRR type DSB repair; a possibility of using BRCA2 siRNA as\nan effective radiosensitizer in tumor radiotherapy may arise.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-9032","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yu, Dong"}],"nameIdentifiers":[{"nameIdentifier":"448234","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sekine, Emiko"}],"nameIdentifiers":[{"nameIdentifier":"448235","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"448236","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ochiya, Takahiro"}],"nameIdentifiers":[{"nameIdentifier":"448237","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"448238","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"于 冬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448239","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"関根 絵美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448240","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448241","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448242","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-04-21"},"publish_date":"2008-04-21","publish_status":"0","recid":"45140","relation_version_is_last":true,"title":["Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:07:12.693316+00:00"}