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Different effects of carbon ion and gamma-irradiation on expression of recepter activator of NF-kB ligand in MC3T3-E1 osteoblast cells
https://repo.qst.go.jp/records/45121
https://repo.qst.go.jp/records/45121af0f622c-4697-4045-bc55-559420ec43eb
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-03-24 | |||||
| タイトル | ||||||
| タイトル | Different effects of carbon ion and gamma-irradiation on expression of recepter activator of NF-kB ligand in MC3T3-E1 osteoblast cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sawajiri, Masahiko
× Sawajiri, Masahiko× Nomura, Yuuji× Kumar, Bhawal Ujjal× Nishikiori, Ryo× Okazaki, Masayuki× Mizoe, Junetsu× Tanimoto, Keiji× 澤尻 昌彦× 野村 雄二× 溝江 純悦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the effects of carbon ion and gamma-irradiation on osteoblastic MC3T3-E1 cells by comparing mRNA expression levels for RANKL and osteoprotegerin by RT-PCR. MC3T3-E1 cells were irradiated with 2, 4, or 6 Gy of carbon ions or gamma-rays, and total RNA was harvested 1, 2, 3, 5, or 7 days after irradiation. The RANKL mRNA/OPG mRNA ratio in carbon ion-irradiated MC3T3-E1 cells was lower, while in gamma-irradiated MC3T3-E1 cells this ratio was higher than in non-irradiated cells. To evaluate osteoclastogenesis of MC3T3-E1 cells, carbon ion- or gamma-irradiated cells were co-cultured with non-irradiated cells from murine bone marrow. Staining for tartrate-resistant acid phosphatase (TRAP) in co-cultures showed that carbon ion irradiation suppressed osteoclastogenesis. This result is consistent with the lower RANKL/OPG mRNA ratio for carbon ion-irradiated cells. These results suggest that carbon ion irradiation acts primarily on osteoblastic cells, leading to a decrease in the RANKL/OPG mRNA ratio. This effect, in turn, leads to a decrease in osteoclastogenesis and osteoclast activity, which results in an increase in bone volume. | |||||
| 書誌情報 |
Bulletin of Experimental Biology and Medicine 巻 142, 号 5, p. 618-624, 発行日 2006-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0007-4888 | |||||
