{"created":"2023-05-15T14:34:54.129720+00:00","id":45121,"links":{},"metadata":{"_buckets":{"deposit":"26eff2a0-2238-4d39-89bc-e20a129e113c"},"_deposit":{"created_by":1,"id":"45121","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45121"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045121","sets":["1"]},"author_link":["448079","448081","448082","448080","448086","448083","448084","448087","448078","448085"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"624","bibliographicPageStart":"618","bibliographicVolumeNumber":"142","bibliographic_titles":[{"bibliographic_title":"Bulletin of Experimental Biology and Medicine"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the effects of carbon ion and gamma-irradiation on osteoblastic MC3T3-E1 cells by comparing mRNA expression levels for RANKL and osteoprotegerin by RT-PCR. MC3T3-E1 cells were irradiated with 2, 4, or 6 Gy of carbon ions or gamma-rays, and total RNA was harvested 1, 2, 3, 5, or 7 days after irradiation. The RANKL mRNA/OPG mRNA ratio in carbon ion-irradiated MC3T3-E1 cells was lower, while in gamma-irradiated MC3T3-E1 cells this ratio was higher than in non-irradiated cells. To evaluate osteoclastogenesis of MC3T3-E1 cells, carbon ion- or gamma-irradiated cells were co-cultured with non-irradiated cells from murine bone marrow. Staining for tartrate-resistant acid phosphatase (TRAP) in co-cultures showed that carbon ion irradiation suppressed osteoclastogenesis. This result is consistent with the lower RANKL/OPG mRNA ratio for carbon ion-irradiated cells. These results suggest that carbon ion irradiation acts primarily on osteoblastic cells, leading to a decrease in the RANKL/OPG mRNA ratio. This effect, in turn, leads to a decrease in osteoclastogenesis and osteoclast activity, which results in an increase in bone volume.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0007-4888","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sawajiri, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"448078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nomura, Yuuji"}],"nameIdentifiers":[{"nameIdentifier":"448079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kumar, Bhawal Ujjal"}],"nameIdentifiers":[{"nameIdentifier":"448080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishikiori, Ryo"}],"nameIdentifiers":[{"nameIdentifier":"448081","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazaki, Masayuki"}],"nameIdentifiers":[{"nameIdentifier":"448082","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizoe, Junetsu"}],"nameIdentifiers":[{"nameIdentifier":"448083","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanimoto, Keiji"}],"nameIdentifiers":[{"nameIdentifier":"448084","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"澤尻 昌彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448085","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野村 雄二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448086","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"溝江 純悦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448087","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Different effects of carbon ion and gamma-irradiation on expression of recepter activator of NF-kB ligand in  MC3T3-E1 osteoblast cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Different effects of carbon ion and gamma-irradiation on expression of recepter activator of NF-kB ligand in  MC3T3-E1 osteoblast cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-03-24"},"publish_date":"2008-03-24","publish_status":"0","recid":"45121","relation_version_is_last":true,"title":["Different effects of carbon ion and gamma-irradiation on expression of recepter activator of NF-kB ligand in  MC3T3-E1 osteoblast cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:07:26.312638+00:00"}