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Globin Gene Enhancer Activity of DNase-I Hypersensitive Site-40 Homolog in Medaka, Oryzias latipes
https://repo.qst.go.jp/records/45007
https://repo.qst.go.jp/records/4500763487798-3ee6-4ee3-b645-a094fde03c15
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-06-07 | |||||
| タイトル | ||||||
| タイトル | Globin Gene Enhancer Activity of DNase-I Hypersensitive Site-40 Homolog in Medaka, Oryzias latipes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Maruyama, Kouichi
× Maruyama, Kouichi× Ishikawa, Yuji× Yasumasu, Shigeki× Iuchi, Ichiro× Kouichi, Maruyama× Yuuji, Ishikawa× Shigeki, Yasumasu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In medaka, we found a C16orf35 gene-like gene at the 1Kbp-3'downstream region of the yb end of the 36Kbp embryonic globin gene cluster (5'a03'-3'b15'-5'a13'-5'b23'-5'a23'-3'a35'-5'b33'-3'b45'-5'a43'-3'ya5'-5'yb3 '). Intron 5 of the gene contained a region having NF-E2 binding sites located between GATA boxes. The region was homologous to human HS-40 in the existence and structure of characteristic transcription factor binding sites and was named Ol-HS-40. Injection of the fusion gene construct (Ol-HS-40-a0(up-2)GFP) consisting of Ol-HS-40, a 200bp-5'upstream minimum promoter for a0, and GFP showed that Ol-HS-40, as in human HS-40, had the ability to strongly enhance GFP expression in erythroid cells of embryos. Further analysis using transgenic technology revealed that Ol-HS-40 had the ability to change the embryo-to-young fish expression of GFP into the embryo-to-adult expression. In addition, the results suggest that Ol-HS-40, although its natural function remains unclear, has the strong enhancer activity for the expression of not only the a-globin gene but also the b-globin gene. |
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| 書誌情報 |
Zoological Science 巻 24, 号 10, p. 997-1004, 発行日 2007-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0289-0003 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | doi:10.2108/zsj.24.997 | |||||
