WEKO3
アイテム
Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA
https://repo.qst.go.jp/records/44982
https://repo.qst.go.jp/records/4498237f5ed97-62de-4052-8bfd-1451f091f61d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-09-25 | |||||
| タイトル | ||||||
| タイトル | Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishii, Nobuyoshi
× Ishii, Nobuyoshi× Sakashita, Tetsuya× Takeda, Hiroshi× Kubota, Yoshihisa× Fuma, Shoichi× Doi, Masahiro× Takahashi, Sentaro× 石井 伸昌× 坂下 哲哉× 武田 洋× 久保田 善久× 府馬 正一× 土居 雅広× 高橋 千太郎 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Extracellular DNA is omnipresent in aquatic environments and is thought to be a genetic material for horizontal gene transformation between microorganisms. We studied the impact of gamma irradiation on the transformation efficiency (transformants number per ng of DNA per ml) of extracellular DNA. Plasmid pEGFP as a model extracellular DNA was irradiated by gamma rays. The transformation efficiency decreased with the increase in radiation dose. A total dose of 10 Gy is normally not lethal for microorganisms but certainly affects the transformation efficiency of extracellular DNA. The decrease in the efficiency would be induced by strand breaks of extracellular DNA because the yield of both single- (SSBs) and double- strand breaks (DSBs) increased with the increase in radiation dose. The relative transformation efficiency of SSBs and DSBs to that of covalently closed circles (CCCs) was 30.3% and 0.2%, respectively. This impact on natural transformation suggests an inability of microorganisms to acquire new characteristics which should be normally acquired. | |||||
| 書誌情報 |
Journal of Environmental Radioactivity 巻 97, 号 2/3, p. 159-167, 発行日 2007-06 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0265-931X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jenvrad.2007.04.002 | |||||
