{"created":"2023-05-15T14:34:47.856384+00:00","id":44982,"links":{},"metadata":{"_buckets":{"deposit":"46b13e77-2926-4d4a-93d0-05f2579023ff"},"_deposit":{"created_by":1,"id":"44982","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44982"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044982","sets":["1"]},"author_link":["446692","446693","446689","446697","446687","446688","446684","446690","446695","446685","446686","446691","446694","446696"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2/3","bibliographicPageEnd":"167","bibliographicPageStart":"159","bibliographicVolumeNumber":"97","bibliographic_titles":[{"bibliographic_title":"Journal of Environmental Radioactivity"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Extracellular DNA is omnipresent in aquatic environments and is thought to be a genetic material for horizontal gene transformation between microorganisms.  We studied the impact of gamma irradiation on the transformation efficiency (transformants number per ng of DNA per ml) of extracellular DNA.  Plasmid pEGFP as a model extracellular DNA was irradiated by gamma rays.  The transformation efficiency decreased with the increase in radiation dose.  A total dose of 10 Gy is normally not lethal for microorganisms but certainly affects the transformation efficiency of extracellular DNA.  The decrease in the efficiency would be induced by strand breaks of extracellular DNA because the yield of both single- (SSBs) and double- strand breaks (DSBs) increased with the increase in radiation dose.  The relative transformation efficiency of SSBs and DSBs to that of covalently closed circles (CCCs) was 30.3% and 0.2%, respectively.  This impact on natural transformation suggests an inability of microorganisms to acquire new characteristics which should be normally acquired.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jenvrad.2007.04.002","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0265-931X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishii, Nobuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"446684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakashita, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"446685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takeda, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"446686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Yoshihisa"}],"nameIdentifiers":[{"nameIdentifier":"446687","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fuma, Shoichi"}],"nameIdentifiers":[{"nameIdentifier":"446688","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Doi, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"446689","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Sentaro"}],"nameIdentifiers":[{"nameIdentifier":"446690","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 伸昌","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂下 哲哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446692","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武田 洋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446693","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保田 善久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446694","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"府馬 正一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446695","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"土居 雅広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446696","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 千太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446697","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-09-25"},"publish_date":"2007-09-25","publish_status":"0","recid":"44982","relation_version_is_last":true,"title":["Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:09:03.591454+00:00"}