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Dual-labeled telomere sensing probes for quantification of telomerase activity assay
https://repo.qst.go.jp/records/44942
https://repo.qst.go.jp/records/4494241588180-41a2-456c-8f4b-1513bae23bbe
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-08-15 | |||||
| タイトル | ||||||
| タイトル | Dual-labeled telomere sensing probes for quantification of telomerase activity assay | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Bakalova-Zheleva, Rumiana
× Bakalova-Zheleva, Rumiana× Zhelev, Zhivko× Kubo, Takanori× Ohba, Hideki× et.al× バカロバ ルミアナ |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The present study describes an empirically discovered phenomenon that might be useful for development of a sensitive and rapid methodology for quantification of telomerase activity assay with simple data acquisition and possibility for calculation of telomerase product in absolute units. The method is based on the design and application of two single-stranded telomere sensing probes consisting of dual-labeled 16-mer oligonucleotides (fluorescent Cy3/Cy3-labeled and non-fluorescent IowaBlack/BHQ-labeled) that can simultaneously hybridize on the primary product of the telomerase reaction. | |||||
| 書誌情報 |
Journal of Biochemical and Biophysical Methods 巻 70, 号 3, p. 503-506, 発行日 2007-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0165-022X | |||||
