{"created":"2023-05-15T14:34:46.120554+00:00","id":44942,"links":{},"metadata":{"_buckets":{"deposit":"6d5bbf08-fe47-4b7f-9c22-03b7b254ea18"},"_deposit":{"created_by":1,"id":"44942","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44942"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044942","sets":["1"]},"author_link":["446313","446312","446311","446315","446310","446314"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"506","bibliographicPageStart":"503","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemical and Biophysical Methods"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study describes an empirically discovered phenomenon that might be useful for development of a sensitive and rapid methodology for quantification of telomerase activity assay with simple data acquisition and possibility for calculation of telomerase product in absolute units. The method is based on the design and application of two single-stranded telomere sensing probes consisting of dual-labeled 16-mer oligonucleotides (fluorescent Cy3/Cy3-labeled and non-fluorescent IowaBlack/BHQ-labeled) that can simultaneously hybridize on the primary product of the telomerase reaction.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0165-022X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Bakalova-Zheleva, Rumiana"}],"nameIdentifiers":[{"nameIdentifier":"446310","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhelev, Zhivko"}],"nameIdentifiers":[{"nameIdentifier":"446311","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubo, Takanori"}],"nameIdentifiers":[{"nameIdentifier":"446312","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohba, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"446313","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"446314","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"バカロバ ルミアナ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446315","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Dual-labeled telomere sensing probes for quantification of telomerase activity assay","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dual-labeled telomere sensing probes for quantification of telomerase activity assay"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-08-15"},"publish_date":"2007-08-15","publish_status":"0","recid":"44942","relation_version_is_last":true,"title":["Dual-labeled telomere sensing probes for quantification of telomerase activity assay"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:09:31.709953+00:00"}