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Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam
https://repo.qst.go.jp/records/44923
https://repo.qst.go.jp/records/449235ee39310-1807-42ba-9ac4-170f4d7e9e85
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-25 | |||||
| タイトル | ||||||
| タイトル | Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tsuboi, Koji
× Tsuboi, Koji× Moritake, Takashi× Yukihiro, Tsuchida× Tokuuye, Koichi× Matsumura, Akira× Ando, Koichi× 坪井 康次× 盛武 敬× 安藤 興一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes of cell inactivation assays. Two human glioblastoma cell lines with or without p53-mutation, and fibroblasts were used as materials. Gamma rays and 290 MeV/u carbon beams with LET values of 20, 40, 80 keV/mum were used. To evaluate cell inactivation, we used colony formation assay, morphological detection of apoptosis, and flow-cytometry. Serial expressions of p53 and p21 were analyzed by immunoblotting. High-LET IR reduced the reproductive potency of these cells to identical levels in spite of differences in gamma-sensitivity, and yield of cell death correlated to LET values. A p53-wild-type glioblastoma cell line demonstrated a higher yield of apoptosis than other cell lines, whereas fibroblasts hardly displayed any cell death indicating senescence-like growth arrest even after high LET IR. A p53-mutant tumor cell line demonstrated very low yield of cell death with prominent G2/M arrest. Results of radiosensitivity differ according to what mode of cell inactivation is selected. While fibroblasts depend on G1 block after IR, G2/M blocks may play crucial roles in the radioresistance of p53-mutant glioblastoma cells. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 48, 号 4, p. 317-325, 発行日 2007-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1269/jrr.06081 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/jrr/48/4/317/_pdf | |||||
| 関連名称 | http://www.jstage.jst.go.jp/article/jrr/48/4/317/_pdf | |||||
