{"created":"2023-05-15T14:34:45.294407+00:00","id":44923,"links":{},"metadata":{"_buckets":{"deposit":"6cdba5a1-9bf6-4b4e-b89c-ce8a0d23ce17"},"_deposit":{"created_by":1,"id":"44923","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44923"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044923","sets":["1"]},"author_link":["446162","446158","446165","446160","446159","446163","446161","446166","446164"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"325","bibliographicPageStart":"317","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes of cell inactivation assays. Two human glioblastoma cell lines with or without p53-mutation, and fibroblasts were used as materials. Gamma rays and 290 MeV/u carbon beams with LET values of 20, 40, 80 keV/mum were used. To evaluate cell inactivation, we used colony formation assay, morphological detection of apoptosis, and flow-cytometry. Serial expressions of p53 and p21 were analyzed by immunoblotting. High-LET IR reduced the reproductive potency of these cells to identical levels in spite of differences in gamma-sensitivity, and yield of cell death correlated to LET values. A p53-wild-type glioblastoma cell line demonstrated a higher yield of apoptosis than other cell lines, whereas fibroblasts hardly displayed any cell death indicating senescence-like growth arrest even after high LET IR. A p53-mutant tumor cell line demonstrated very low yield of cell death with prominent G2/M arrest. Results of radiosensitivity differ according to what mode of cell inactivation is selected. While fibroblasts depend on G1 block after IR, G2/M blocks may play crucial roles in the radioresistance of p53-mutant glioblastoma cells.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1269/jrr.06081","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.jstage.jst.go.jp/article/jrr/48/4/317/_pdf"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jstage.jst.go.jp/article/jrr/48/4/317/_pdf","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tsuboi, Koji"}],"nameIdentifiers":[{"nameIdentifier":"446158","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Moritake, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"446159","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yukihiro, Tsuchida"}],"nameIdentifiers":[{"nameIdentifier":"446160","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokuuye, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"446161","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumura, Akira"}],"nameIdentifiers":[{"nameIdentifier":"446162","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ando, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"446163","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坪井 康次","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446164","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"盛武 敬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446165","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安藤 興一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446166","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-07-25"},"publish_date":"2007-07-25","publish_status":"0","recid":"44923","relation_version_is_last":true,"title":["Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:09:44.871846+00:00"}