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Formation of an Active Form of the Interleukin-2/15 Receptor beta-Chain by Insertion of the Intracisternal A Particle in a Radiation-Induced Mouse Thymic Lymphoma and Its Role in Tumorigenesis
https://repo.qst.go.jp/records/44526
https://repo.qst.go.jp/records/44526530a592f-f9fe-45af-8aca-60694c8a917c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-08-03 | |||||
| タイトル | ||||||
| タイトル | Formation of an Active Form of the Interleukin-2/15 Receptor beta-Chain by Insertion of the Intracisternal A Particle in a Radiation-Induced Mouse Thymic Lymphoma and Its Role in Tumorigenesis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ukai, Hideki
× Ukai, Hideki× Ishii-Ohba, Hiroko× Ukai-tadenuma, Maki× Ogiu, Toshiaki× Tsuji, Hideo× 鵜飼 英樹× 石井 洋子× 荻生 俊昭× 辻 秀雄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although many reports suggest that aberrant regulation of cytokine signaling pathways via the interleukin-2 receptor (IL-2R) induces tumorigenic transformation, constitutively active IL-2R in tumors has not been reported. We searched for genomic alteration of the IL-2/15R beta-subunit gene (IL-2/15R beta) in cytokine-independent cell lines established from radiation-induced mouse thymic lymphomas. In the TL34 cell line and its primary tumor, one of the IL-2/15R beta alleles was rearranged by the insertion of an intracisternal A particle (IAP) retrotransposon. The IAP-IL2/15R beta chimeric gene expressed chimeric mRNA in which IAP-coding Gag-Pol mRNA was fused to IL-2/15R beta mRNA and coded for Gag-Pol-IL-2/15R beta chimeric protein. Forced expression of the Gag-Pol-IL-2/15R beta chimeric cDNA in a mouse cytotoxic T-cell line (CTLL-2) converted IL-2-dependent cell growth to IL-2-independent growth, suggesting that the chimeric protein activates some of the IL-2 signaling pathways necessary for cell proliferation. Downregulation of the expression of the Gag-Pol-IL-2/15R beta chimeric protein in TL34 by antisense RNA inhibited cell growth, and concomitantly reduced the level of c-myc protein. These results suggest that the Gag-Pol-IL-2/15R beta is a constitutively active form that transmits proliferative signals by expressing downstream target genes, including c-myc. Thus, we demonstrated that the chimeric receptor gene produced by the insertion of an IAP functions as an oncogene by providing IL-2-independent autonomous growth potential. | |||||
| 書誌情報 |
Molecular Carcinogenesis 巻 37, p. 110-119, 発行日 2003 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0899-1987 | |||||
