{"created":"2023-05-15T14:34:27.760205+00:00","id":44526,"links":{},"metadata":{"_buckets":{"deposit":"c685cc44-04c9-49fc-a11a-faefa529c7fa"},"_deposit":{"created_by":1,"id":"44526","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44526"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044526","sets":["1"]},"author_link":["442439","442438","442436","442443","442442","442441","442437","442440","442435"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"119","bibliographicPageStart":"110","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Molecular Carcinogenesis"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although many reports suggest that aberrant regulation of cytokine signaling pathways via the interleukin-2 receptor (IL-2R) induces tumorigenic transformation, constitutively active IL-2R in tumors has not been reported. We searched for genomic alteration of the IL-2/15R beta-subunit gene (IL-2/15R beta) in cytokine-independent cell lines established from radiation-induced mouse thymic lymphomas. In the TL34 cell line and its primary tumor, one of the IL-2/15R beta alleles was rearranged by the insertion of an intracisternal A particle (IAP) retrotransposon. The IAP-IL2/15R beta chimeric gene expressed chimeric mRNA in which IAP-coding Gag-Pol mRNA was fused to IL-2/15R beta mRNA and coded for Gag-Pol-IL-2/15R beta chimeric protein. Forced expression of the Gag-Pol-IL-2/15R beta chimeric cDNA in a mouse cytotoxic T-cell line (CTLL-2) converted IL-2-dependent cell growth to IL-2-independent growth, suggesting that the chimeric protein activates some of the IL-2 signaling pathways necessary for cell proliferation. Downregulation of the expression of the Gag-Pol-IL-2/15R beta chimeric protein in TL34 by antisense RNA inhibited cell growth, and concomitantly reduced the level of c-myc protein. These results suggest that the Gag-Pol-IL-2/15R beta is a constitutively active form that transmits proliferative signals by expressing downstream target genes, including c-myc. Thus, we demonstrated that the chimeric receptor gene produced by the insertion of an IAP functions as an oncogene by providing IL-2-independent autonomous growth potential.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0899-1987","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ukai, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"442435","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii-Ohba, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"442436","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ukai-tadenuma, Maki"}],"nameIdentifiers":[{"nameIdentifier":"442437","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogiu, Toshiaki"}],"nameIdentifiers":[{"nameIdentifier":"442438","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Hideo"}],"nameIdentifiers":[{"nameIdentifier":"442439","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鵜飼 英樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442440","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 洋子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442441","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荻生 俊昭","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442442","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442443","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Formation of an Active Form of the Interleukin-2/15 Receptor beta-Chain by Insertion of the Intracisternal A Particle in a Radiation-Induced Mouse Thymic Lymphoma and Its Role in Tumorigenesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Formation of an Active Form of the Interleukin-2/15 Receptor beta-Chain by Insertion of the Intracisternal A Particle in a Radiation-Induced Mouse Thymic Lymphoma and Its Role in Tumorigenesis"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-08-03"},"publish_date":"2006-08-03","publish_status":"0","recid":"44526","relation_version_is_last":true,"title":["Formation of an Active Form of the Interleukin-2/15 Receptor beta-Chain by Insertion of the Intracisternal A Particle in a Radiation-Induced Mouse Thymic Lymphoma and Its Role in Tumorigenesis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:14:26.132150+00:00"}