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Labeling of Phosphorothioate Antisense Oligonucleotides with Yttrium-90
https://repo.qst.go.jp/records/44084
https://repo.qst.go.jp/records/440849efd1a71-7a13-4527-be84-e2f9247002a0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-05-22 | |||||
| タイトル | ||||||
| タイトル | Labeling of Phosphorothioate Antisense Oligonucleotides with Yttrium-90 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Watanabe, Naoyuki
× Watanabe, Naoyuki× Endou, Keigo× Tanada, Shuji× Murata, Hajime× Sasaki, Yasuhito× et.al× 渡辺 直行× 遠藤 啓吾× 棚田 修二× 村田 啓× 佐々木 康人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Novel yttrium-90 (90Y)-labeled phosphorothioate antisense oligonucleotides were designed as a potential targeted radionuclede therapeutic agent for malignant tumors. A 15-mer phosphorothioate antisense oligonucleotide, which was complementary to the translation start region of the N-myc oncogene mRNA, was conjugated with isothiocyanobenzyl ethylenediamine tetraacetic acid (SCN-Bn-EDTA), via a C-5-substituted deoxyuridine that had replaced a thymine in the oligonucleotide, and wa then labeled with 90Y-acetate. Following purification, the radiochemical purity of hte 90Y-Bn-EDTA-phosphorothioate antisence oligonucleotides was estimated by 2.0% agarose gel electrophoresis, and the specific hybridization of 90Y-Bn-EDTA-phosphorothioate antisense oligonucleotide to a phosphorodiester sense oligonucleotide was investigated by 20% polyacrylamide gel electrophoresis in a cell-free system. Radiochemical purity was 98.7 0.4% at 72 h after labeling and 90.3 0.9% after 72-h incubation with human normal serum. The 90Y-Bn-EDTA-phosphorothioate antisense oligonucleotide hybridized specifically to a complementary phosphorodiester sense oligonucleotide. In conclusion, phosphorothioate antisense oligonucleotides can be labeled stably with 90Y using SCN-Bn-EDTA without loss of hybridization properties. | |||||
| 書誌情報 |
Nuclear Medicine and Biology 巻 26, 号 2, p. 239-243, 発行日 1999 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-8051 | |||||
