{"created":"2023-05-15T14:33:59.990792+00:00","id":44084,"links":{},"metadata":{"_buckets":{"deposit":"21e2f9d3-c254-493e-9d5b-4219c3ea4295"},"_deposit":{"created_by":1,"id":"44084","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44084"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044084","sets":["1"]},"author_link":["438239","438246","438244","438242","438245","438243","438238","438237","438236","438240","438241"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"243","bibliographicPageStart":"239","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Novel yttrium-90 (90Y)-labeled phosphorothioate antisense oligonucleotides were designed as a potential targeted radionuclede therapeutic agent for malignant tumors.  A 15-mer phosphorothioate antisense oligonucleotide, which was complementary to the translation start region of the N-myc oncogene mRNA, was conjugated with isothiocyanobenzyl ethylenediamine tetraacetic acid (SCN-Bn-EDTA), via a C-5-substituted deoxyuridine that had replaced a thymine in the oligonucleotide, and wa then labeled with 90Y-acetate. Following purification, the radiochemical purity of hte 90Y-Bn-EDTA-phosphorothioate antisence oligonucleotides was estimated by 2.0% agarose gel electrophoresis, and the specific hybridization of 90Y-Bn-EDTA-phosphorothioate antisense oligonucleotide to a phosphorodiester sense oligonucleotide was investigated by 20% polyacrylamide gel electrophoresis in a cell-free system. Radiochemical purity was 98.7   0.4% at 72 h after labeling and 90.3   0.9% after 72-h incubation with human normal serum.  The 90Y-Bn-EDTA-phosphorothioate antisense oligonucleotide hybridized specifically to a complementary phosphorodiester sense oligonucleotide.  In conclusion, phosphorothioate antisense oligonucleotides can be labeled stably with 90Y using SCN-Bn-EDTA without loss of hybridization properties.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Watanabe, Naoyuki"}],"nameIdentifiers":[{"nameIdentifier":"438236","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Endou, Keigo"}],"nameIdentifiers":[{"nameIdentifier":"438237","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanada, Shuji"}],"nameIdentifiers":[{"nameIdentifier":"438238","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murata, Hajime"}],"nameIdentifiers":[{"nameIdentifier":"438239","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki, Yasuhito"}],"nameIdentifiers":[{"nameIdentifier":"438240","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"438241","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"渡辺 直行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438242","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"遠藤 啓吾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"棚田 修二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村田 啓","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木 康人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438246","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Labeling of Phosphorothioate Antisense Oligonucleotides with Yttrium-90","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Labeling of Phosphorothioate Antisense Oligonucleotides with Yttrium-90"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-05-22"},"publish_date":"2006-05-22","publish_status":"0","recid":"44084","relation_version_is_last":true,"title":["Labeling of Phosphorothioate Antisense Oligonucleotides with Yttrium-90"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:19:37.428235+00:00"}