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Radio-sensitive murine thymoma cell line 3SB: characterization of its apoptosis-resistant variants induced by repeated X-irradiation.
https://repo.qst.go.jp/records/43915
https://repo.qst.go.jp/records/43915c8b8129d-19a8-4ac6-9c48-5669b2821445
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-12-16 | |||||
| タイトル | ||||||
| タイトル | Radio-sensitive murine thymoma cell line 3SB: characterization of its apoptosis-resistant variants induced by repeated X-irradiation. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hama-Inaba, Hiroko
× Hama-Inaba, Hiroko× Bing, Wang× Mori, Masahiko× Matsushima, Tadashi× Saito, Toshiyuki× Takusagawa, Mitsuko× Yamada, Takeshi× Muto, Masahiro× Ohyama, Harumi× 稲葉 浩子× 王 冰× 森 雅彦× 齋藤 俊行× 田草川 光子× 武藤 正弘× 大山 ハルミ |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 3SB, a mouse thymoma cell line, is one of the most radio-sensitive cells (D0 = 0.3 Gy), and its rapid apoptosis (4 h after 5 Gy irradiation, 90% apoptosis) seems to play a decisive role in enhancing the radiosensitivity. To understand the molecular mechanisms underlying extremely high radiosensitivity and rapid apoptosis, we attempted to isolate X-ray-resistant (XR) variants from 3SBH5, a stable subclone of 3SB, by repeating exposure of the cells to 2-5 Gy X-rays. Four independent stable XR variants, R111, R223, R316 and R429, were isolated by the repeated irradiation protocols. All XR cells possessed about 3 times higher D10 values than that of their parental 3SBH5. They were also resistant to apoptosis; only 10% cells underwent apoptosis 4 h after 5 Gy irradiation. The p53 protein was induced in all the cell lines after 5 Gy X-irradiation. These variants showed a cross resistance to a chemical reagent daunorubicin (DNR) that is known to be involved in the ceramide-mediated apoptosis. DNR, as well as C2-ceramide (5 muM) induced apoptosis in parental 3SBH5 cell, but not in two XR variants, R233 and R316 cells. Present result suggests that the induction of X-ray resistance by repeated X-irradiation might be achieved, at least partly, by the enhanced resistance to the ceramide-mediated apoptosis. | |||||
| 書誌情報 |
Fundamental and Molecular Mechanisms of Mutagenesis : A Section of Mutation Research 巻 403, p. 85-94, 発行日 1998-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-5107 | |||||
