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Apoptosis induced by high-LET radiations is not affected by cellular p53 gene status
https://repo.qst.go.jp/records/43864
https://repo.qst.go.jp/records/438642ad48506-d3b2-4edb-a9f1-5db5f6d25bbc
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-12-08 | |||||
| タイトル | ||||||
| タイトル | Apoptosis induced by high-LET radiations is not affected by cellular p53 gene status | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takahashi, Akihisa
× Takahashi, Akihisa× Matsumoto, Hideki× Furusawa, Yoshiya× Ohnishi, Ken× Ohnishi, Takeo× et.al× 高橋 昭久× 松本 英樹× 古澤 佳也× 大西 健× 大西 武雄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To learn more about the biological effects of high-linear energy transfer (LET) radiations, we examined radiation-induced apoptosis in response to high-LET radiations in cells with wild-type, mutated and null p53 gene. Three human lung cancer cell lines were used. These lines had identical genotypes, except for the p53 gene. Cells were exposed to X-rays or high-LET radiations (13 – 200 keV mm71) using different nuclei ion beams. Cellular radiation sensitivities were determined with the use of colony-forming assays. Apoptosis was detected and quantified using Hoechst 33342 staining with fluorescence microscopy. It was found that (1) there was no significant difference in cellular sensitivity to high-LET radiation (485 keV/um), although the sensitivity of wild-type p53 cells to X-rays was higher than that of mutated p53 or p53-null cells; (2) X-ray-induced apoptosis at higher frequencies in wild-type p53 cells when compared with mutated p53 and p53-null cells; and (3) Fe beams (200 keV mm71) induced apoptosis in a p53-independent manner. The results indicate that high-LET radiations induces apoptosis in human lung cancer cells in a manner that does not seem to depend on the p53 gene status of the cells. | |||||
| 書誌情報 |
International Journal of Radiation Biology 巻 81, 号 8, p. 581-586, 発行日 2005-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0955-3002 | |||||
