{"created":"2023-05-15T14:33:48.850612+00:00","id":43864,"links":{},"metadata":{"_buckets":{"deposit":"60d2211d-5a65-4894-88c3-2c68706e95ef"},"_deposit":{"created_by":1,"id":"43864","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43864"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043864","sets":["1"]},"author_link":["436109","436106","436110","436105","436102","436104","436103","436108","436101","436107","436111"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"586","bibliographicPageStart":"581","bibliographicVolumeNumber":"81","bibliographic_titles":[{"bibliographic_title":"International Journal of Radiation Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To learn more about the biological effects of high-linear energy transfer (LET) radiations, we examined radiation-induced apoptosis in response to high-LET radiations in cells with wild-type, mutated and null p53 gene. Three human lung cancer cell lines were used. These lines had identical genotypes, except for the p53 gene. Cells were exposed to X-rays or high-LET radiations (13 – 200 keV mm71) using different nuclei ion beams. Cellular radiation sensitivities were determined with the use of colony-forming assays. Apoptosis was detected and quantified using Hoechst 33342 staining with fluorescence microscopy. It was found that (1) there was no significant difference in cellular sensitivity to high-LET radiation (485 keV/um), although the sensitivity of wild-type p53 cells to X-rays was higher than that of mutated p53 or p53-null cells; (2) X-ray-induced apoptosis at higher frequencies in wild-type p53 cells when compared with mutated p53 and p53-null cells; and (3) Fe beams (200 keV mm71) induced apoptosis in a p53-independent manner. The results indicate that high-LET radiations induces apoptosis in human lung cancer cells in a manner that does not seem to depend on the p53 gene status of the cells.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0955-3002","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Akihisa"}],"nameIdentifiers":[{"nameIdentifier":"436101","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"436102","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"436103","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohnishi, Ken"}],"nameIdentifiers":[{"nameIdentifier":"436104","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohnishi, Takeo"}],"nameIdentifiers":[{"nameIdentifier":"436105","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"436106","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 昭久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"436107","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 英樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"436108","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"436109","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 健","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"436110","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 武雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"436111","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Apoptosis induced by high-LET radiations is not affected by cellular p53 gene status","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Apoptosis induced by high-LET radiations is not affected by cellular p53 gene status"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-12-08"},"publish_date":"2005-12-08","publish_status":"0","recid":"43864","relation_version_is_last":true,"title":["Apoptosis induced by high-LET radiations is not affected by cellular p53 gene status"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:22:10.257846+00:00"}