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Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts.
https://repo.qst.go.jp/records/43469
https://repo.qst.go.jp/records/43469b0dc8c9e-1276-44da-bac4-9c27b1ccbad0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-08-26 | |||||
| タイトル | ||||||
| タイトル | Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kawata, Tetsuya
× Kawata, Tetsuya× Durante, Marco× Furusawa, Yoshiya× George, Kerry× Ito, Hisao× Wu, Honglu× A, Cucinotta Francesca× 川田 哲也× 古澤 佳也× ジョージ ケリー× 伊東 久夫× ウー ホングル× クシノッタ フランク |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We reported previously that exposure of normal human fibroblasts in G2 phase of the cell cycle to high-LET radiation produces a much higher frequency of isochromatid breaks than exposure to gamma rays. We concluded that an increase in the production of isochromatid breaks is a signature of initial high-LET radiation-induced G2-phase damage. In this paper, we report the repair kinetics of isochromatid breaks induced by high-LET radiation in normal G2-phase human fibroblasts. Exponentially growing human fibroblasts (AG1522) were irradiated with gamma rays or energetic carbon (290 MeV/nucleon), silicon (490 MeV/nucleon), or iron (200 MeV/nucleon) ions. Prematurely condensed chromosomes were induced by calyculin A after different postirradiation incubation times ranging from 0 to 600 min. Chromosomes were stained with Giemsa, and aberrations were scored in cells at G2 phase. G2-phase fragments, the result of the induction of isochromatid breaks, decreased quickly with incubation time. The curve for the kinetics of the rejoining of chromatid-type breaks showed a slight upward curvature with time after exposure to 440 keV/microm iron particles, probably due to isochromatid-isochromatid break rejoining. The formation of chromatid exchanges after exposure to high-LET radiation therefore appears to be underestimated, because isochromatid-isochromatid exchanges cannot be detected. Increased induction of isochromatid breaks and rejoining of isochromatid breaks affect the overall kinetics of chromatid-type break rejoining after exposure to high-LET radiation. | |||||
| 書誌情報 |
Radiation Research 巻 156, 号 5, p. 598-602, 発行日 2001-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
