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  1. 原著論文

The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif.

https://repo.qst.go.jp/records/43295
https://repo.qst.go.jp/records/43295
b191a721-fbdd-44e5-a52a-5a231f5a4090
Item type 学術雑誌論文 / Journal Article(1)
公開日 2003-12-05
タイトル
タイトル The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif.
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Fukuchi, Kunihiko

× Fukuchi, Kunihiko

WEKO 430670

Fukuchi, Kunihiko

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Nakamura, Kenntarou

× Nakamura, Kenntarou

WEKO 430671

Nakamura, Kenntarou

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Ichimura, Sachiko

× Ichimura, Sachiko

WEKO 430672

Ichimura, Sachiko

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Tatsumi, Kouichi

× Tatsumi, Kouichi

WEKO 430673

Tatsumi, Kouichi

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Gomi, Kunihide

× Gomi, Kunihide

WEKO 430674

Gomi, Kunihide

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沼田 幸子

× 沼田 幸子

WEKO 430675

en 沼田 幸子

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巽 紘一

× 巽 紘一

WEKO 430676

en 巽 紘一

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抄録
内容記述タイプ Abstract
内容記述 The cyclin kinase inhibitor p21 associates with and inhibits cyclin-CDKs to retard the progress of the cell cycle in response to DNA damage. The recognition sites for cyclin binding on the various cell cycle-related molecules have been identified as RXL motifs. In the case of p21, the dependence of the Cy1(18CRRL) or Cy2(154KRRL) motifs on cyclin E, but not on cyclin A has been demonstrated by in vitro experiments. In this study, to clarify the mechanism of p21 association with cyclin A, we constructed a p21 expression system in mammalian cells. After transfection with an expression vector containing cDNA of various p21-mutants, cells were irradiated with 10Gy of gamma-rays to introduce DNA damage, followed by quantification of the p21-cyclin A association. The p21-mutant constructs were single or multiple deletions in Cy1, Cy2, and the CDK2 binding region, and a nonphosphorylatable alanine mutant of the C-terminal phosphorylation site. We demonstrated that the association of p21 and cyclin A in response to gamma-irradiation requires the CDK binding region, 49-71 aa, but not the Cy motifs. We believe the mechanism by which p21 inhibits cyclin-CDKs is distinct in each phase of the cell cycle. Furthermore, the increase in the asoociation of p21 and cyclin A was not correlated with the levels of p21. This suggests that DNA damage triggers a signal to the p21 region between 21 and 96 as to allow cyclin A association.
書誌情報 Biochimica et Biophysica Acta. Molecular Cell Research

巻 1642, p. 163-171, 発行日 2003
ISSN
収録物識別子タイプ ISSN
収録物識別子 0167-4889
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