{"created":"2023-05-15T14:33:23.104601+00:00","id":43295,"links":{},"metadata":{"_buckets":{"deposit":"2e1f2efb-452d-41a4-9369-20ebd36e3aac"},"_deposit":{"created_by":1,"id":"43295","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43295"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043295","sets":["1"]},"author_link":["430674","430673","430670","430676","430671","430672","430675"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"171","bibliographicPageStart":"163","bibliographicVolumeNumber":"1642","bibliographic_titles":[{"bibliographic_title":"Biochimica et Biophysica Acta. Molecular Cell Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The cyclin kinase inhibitor p21 associates with and inhibits cyclin-CDKs to retard the progress of the cell cycle in response to DNA damage. The recognition sites for cyclin binding on the various cell cycle-related molecules have been identified as RXL motifs. In the case of p21, the dependence of the Cy1(18CRRL) or Cy2(154KRRL) motifs on cyclin E, but not on cyclin A has been demonstrated by in vitro experiments. In this study, to clarify the mechanism of p21 association with cyclin A, we constructed a p21 expression system in mammalian cells. After transfection with an expression vector containing cDNA of various p21-mutants, cells were irradiated with 10Gy of gamma-rays to introduce DNA damage, followed by quantification of the p21-cyclin A association. The p21-mutant constructs were single or multiple deletions in Cy1, Cy2, and the CDK2 binding region, and a nonphosphorylatable alanine mutant of the C-terminal phosphorylation site. We demonstrated that the association of p21 and cyclin A in response to gamma-irradiation requires the CDK binding region, 49-71 aa, but not the Cy motifs. We believe the mechanism by which p21 inhibits cyclin-CDKs is distinct in each phase of the cell cycle. Furthermore, the increase in the asoociation of p21 and cyclin A was not correlated with the levels of p21. This suggests that DNA damage triggers a signal to the p21 region between 21 and 96 as to allow cyclin A association.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0167-4889","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukuchi, Kunihiko"}],"nameIdentifiers":[{"nameIdentifier":"430670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Kenntarou"}],"nameIdentifiers":[{"nameIdentifier":"430671","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichimura, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"430672","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tatsumi, Kouichi"}],"nameIdentifiers":[{"nameIdentifier":"430673","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gomi, Kunihide"}],"nameIdentifiers":[{"nameIdentifier":"430674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"沼田 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽 紘一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430676","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-12-05"},"publish_date":"2003-12-05","publish_status":"0","recid":"43295","relation_version_is_last":true,"title":["The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:29:00.629041+00:00"}