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Genetic susceptibility to thymic lymphomas and K-ras gene mutation in mice after exposure to X-rays and N-ethyl-N-nitrosourea
https://repo.qst.go.jp/records/43220
https://repo.qst.go.jp/records/4322076ff521e-976c-4c26-9c95-398e49ad70e1
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-10-22 | |||||
| タイトル | ||||||
| タイトル | Genetic susceptibility to thymic lymphomas and K-ras gene mutation in mice after exposure to X-rays and N-ethyl-N-nitrosourea | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shimada, Yoshiya
× Shimada, Yoshiya× Nishimura, Mayumi× Kakinuma, Shizuko× Ogiu, Toshiaki× Fujimoto, Hirokazu× Kubo, Ayumi× Nagai, Junya× Tano, Keizou× Yoshinaga, Shinji× 島田 義也× 西村 まゆみ× 柿沼 志津子× 荻生 俊昭× 久保 歩× 永井 絢也× 吉永 信治 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: Ras activation is one of the major mechanisms for the development of murine thymic lymphomas by radiation and chemical carcinogens. To gain insight into the relationship between genetic susceptibility and ras gene mutation, the frequency and spectrum of ras gene mutation was examined in thymic lymphomas from susceptible and resistant mice. Materials and methods: K- and N-ras mutations in thymic lymphomas that arose in X-ray-irradiated and N-ethyl-N-nitrosourea (ENU)-treated mice of susceptible C57BL/6, rather resistant C3H, and their hybrid B6C3F1, were analyzed by PCR-SSCP and subsequent DNA sequencing. Results: C57BL/6 exhibited a higher incidence of thymic lymphomas after exposure to X-rays and ENU than C3H, with B6C3F1 being intermediate. K-ras gene mutations occurred frequently in the pathogenesis of ENU-induced thymic lymphomas in susceptible C57BL/6 as opposed to resistant C3H. The ras mutations were more frequent in ENU-induced thymic lymphomas than X-ray-induced thymic lymphomas and with the latter, there was no clear evidence for strain differences, suggesting that the genetic susceptibility to X-rays was independent of ras activation. The mutations of K-ras in thymic lymphomas from C57BL/6 were predominantly GGT-to-GAT in codon 12, whereas this mutation type was never found in those from C3H. No strain difference was observed in the nucleotide sequence or expression levels of O6-alkylguanine alkyltransferase, indicating that the enzyme did not account for the genetic susceptibility to ras activation. Conclusions: The results indicate that there is a clear strain- and carcinogen-dependency of K-ras mutation, and that the frequency of ras mutation might determine the genetic susceptibility to ENU-induced lymphomagenesis, whereas the pathways(s) independent of ras activation might determine the susceptibility to X-ray-induced lymphomagenesis. |
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| 書誌情報 |
International Journal of Radiation Biology 巻 79, p. 423-430, 発行日 2003-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0955-3002 | |||||
