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Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human Cells for radiation-induced chromosome damage, cell killing and mutation
https://repo.qst.go.jp/records/43138
https://repo.qst.go.jp/records/43138cc8f7aa8-487d-4e1b-ab02-dd7948a1e998
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-25 | |||||
| タイトル | ||||||
| タイトル | Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human Cells for radiation-induced chromosome damage, cell killing and mutation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Peng, Yuanlin
× Peng, Yuanlin× Zhang, Qinming× Nagasawa, Hatsumi× Okayasu, Ryuichi× L., Liber Howard× S., Bedford Joel× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Targeted gene silencing in mammalian cells by RNA interference (RNAi) using small interfering RNAs (siRNAs) was recently described by Elbashir et al. (S.M. Elbashir et al., Nature (Lond.), 411: 494-498, 2001). We have used this methodology in several human cell strains to reduce expression of Prkdc (DNA-PKcs) gene coding for the catalytic sub-unit of the DNA-dependent protein kinase (DNA-PKcs), that is involved in the nonhomologous end joining (NHEJ) of DNA double strand breaks. We have also demonstrated a radiosensitization for several phenotypic endpoints of radiation damage. In low passage normal human fibroblasts, siRNA knock-down of DNA-PKcs resulted in a reduced capacity for restitution of radiation-induced interphase chromosome breaks as measured by premature chromosome condensation (PCC), an increased yield of acentric chromosome fragments at the first post-irradiation mitosis, and an increased radiosensitivity for cell killing. For three strains of related human lymphoblasts, DNA-PKcs targeted siRNA transfection resulted in little or no increase in radiosensitivity with respect to cell killing, a 1.5-fold decrease in induced mutant yield in TK6 and p53-null NH32 cells, but about a 2-fold increase in induced mutant yield in p53 mutant WTK1 cells at both the hypoxanthine quinine phosphoribosyl transferase (hprt) and the thymidine kinase loci. | |||||
| 書誌情報 |
Cancer Research 巻 62, p. 6400-6404, 発行日 2002-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0008-5472 | |||||
