{"created":"2023-05-15T14:33:16.076826+00:00","id":43138,"links":{},"metadata":{"_buckets":{"deposit":"4124d99f-2af3-4a2d-97fa-194612c3a446"},"_deposit":{"created_by":1,"id":"43138","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43138"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043138","sets":["1"]},"author_link":["429187","429192","429189","429190","429191","429188","429193"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6404","bibliographicPageStart":"6400","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"Cancer Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Targeted gene silencing in mammalian cells by RNA interference (RNAi) using small interfering RNAs (siRNAs) was recently described by Elbashir et al. (S.M. Elbashir et al., Nature (Lond.), 411: 494-498, 2001).  We have used this methodology in several human cell strains to reduce expression of Prkdc (DNA-PKcs) gene coding for the catalytic sub-unit of the DNA-dependent protein kinase (DNA-PKcs), that is involved in the nonhomologous end joining (NHEJ) of DNA double strand breaks.  We have also demonstrated a radiosensitization for several phenotypic endpoints of radiation damage.  In low passage normal human fibroblasts, siRNA knock-down of DNA-PKcs resulted in a reduced capacity for restitution of radiation-induced interphase chromosome breaks as measured by premature chromosome condensation (PCC), an increased yield of acentric chromosome fragments at the first post-irradiation mitosis, and an increased radiosensitivity for cell killing.  For three strains of related human lymphoblasts, DNA-PKcs targeted siRNA transfection resulted in little or no increase in radiosensitivity with respect to cell killing, a 1.5-fold decrease in induced mutant yield in TK6 and p53-null NH32 cells, but about a 2-fold increase in induced mutant yield in p53 mutant WTK1 cells at both the hypoxanthine quinine phosphoribosyl transferase (hprt) and the thymidine kinase loci.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0008-5472","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Peng, Yuanlin"}],"nameIdentifiers":[{"nameIdentifier":"429187","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Qinming"}],"nameIdentifiers":[{"nameIdentifier":"429188","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagasawa, Hatsumi"}],"nameIdentifiers":[{"nameIdentifier":"429189","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"429190","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"L., Liber Howard"}],"nameIdentifiers":[{"nameIdentifier":"429191","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"S., Bedford Joel"}],"nameIdentifiers":[{"nameIdentifier":"429192","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"429193","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human Cells for radiation-induced chromosome damage, cell killing and mutation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human Cells for radiation-induced chromosome damage, cell killing and mutation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-08-25"},"publish_date":"2003-08-25","publish_status":"0","recid":"43138","relation_version_is_last":true,"title":["Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human Cells for radiation-induced chromosome damage, cell killing and mutation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:30:49.029096+00:00"}