WEKO3
アイテム
{"_buckets": {"deposit": "9967669d-754e-48e0-a19d-c585d280397b"}, "_deposit": {"created_by": 1, "id": "69752", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "69752"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00069752", "sets": ["28"]}, "author_link": ["684793", "684797", "684792", "684798", "684791", "684794", "684790", "684795", "684799", "684796"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2009-05-20", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Purpose: To investigate cellular responses in normal human fibroblasts induced with low dose irradiations of qualitatively different radiation types. \nMethods: Normal human fibroblasts were pre-treated with low-fluence irradiations (~1mGy/7-8h) of 137Cs gamma rays, 241Am-Be neutrons (maximum energy ; 11.5MeV, average energy ; 5.0MeV), helium (LET=2.3keV/\u0026#181;m), carbon (LET=13.3keV/\u0026#181;m) and iron ions (LET=200keV/\u0026#181;m) before applying irradiation with a 200kV X-ray challenging dose (1.5Gy). The 3 different ion beams were produced by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences. \nResults: There was no difference in X-ray-induced mutation frequency, which was detected with hprt locus detected as 6-thioguanine resistant clones, between un-pretreated and gamma-ray pre-treated cells. In the case of the pre-treatment with high-LET ions, mutation frequency was around 4.0 times higher in carbon- and 1.9 times higher in helium-ion pre-treated cells than in un-pretreated cells, while no difference was observed in iron-ion pre-treated cells. On the contrary, mutation frequency in cells pre-treated with neutrons was reduced at 15% of un-pretreated cells. To identify possible mechanism(s) of the observed cellular response, cells were treated with a specific inhibitor of gap-junction mediated cell-cell communication. The results clearly showed that the observed enhancement of X-ray-induced mutation frequency in cells pre-treated with helium and carbon ions was reduced at the control level and reduction in cells pre-treated with neutrons was came back to the control level. \nConclusion: There is evidence that gap-junction mediated cell-cell communication play an important role of inducing different cellular responses by pre-treatment with different radiation types.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "第二回アジア放射線連合会議(2nd Asian Congress of Radiation Research)", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Suzuki, Masao"}], "nameIdentifiers": [{"nameIdentifier": "684790", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuruoka, Chizuru"}], "nameIdentifiers": [{"nameIdentifier": "684791", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitamura, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "684792", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hua, Liu Cui"}], "nameIdentifiers": [{"nameIdentifier": "684793", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uchihori, Yukio"}], "nameIdentifiers": [{"nameIdentifier": "684794", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鈴木 雅雄", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "684795", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鶴岡 千鶴", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "684796", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "北村 尚", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "684797", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "劉 翠華", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "684798", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "内堀 幸夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "684799", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/69752", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-05-22"}, "publish_date": "2009-05-22", "publish_status": "0", "recid": "69752", "relation": {}, "relation_version_is_last": true, "title": ["Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types"], "weko_shared_id": -1}
Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types
https://repo.qst.go.jp/records/69752
https://repo.qst.go.jp/records/6975255e083e3-0bbc-473b-807b-240079a0f1bf
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2009-05-22 | |||||
タイトル | ||||||
タイトル | Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Suzuki, Masao
× Suzuki, Masao× Tsuruoka, Chizuru× Kitamura, Hisashi× Hua, Liu Cui× Uchihori, Yukio× 鈴木 雅雄× 鶴岡 千鶴× 北村 尚× 劉 翠華× 内堀 幸夫 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: To investigate cellular responses in normal human fibroblasts induced with low dose irradiations of qualitatively different radiation types. Methods: Normal human fibroblasts were pre-treated with low-fluence irradiations (~1mGy/7-8h) of 137Cs gamma rays, 241Am-Be neutrons (maximum energy ; 11.5MeV, average energy ; 5.0MeV), helium (LET=2.3keV/µm), carbon (LET=13.3keV/µm) and iron ions (LET=200keV/µm) before applying irradiation with a 200kV X-ray challenging dose (1.5Gy). The 3 different ion beams were produced by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences. Results: There was no difference in X-ray-induced mutation frequency, which was detected with hprt locus detected as 6-thioguanine resistant clones, between un-pretreated and gamma-ray pre-treated cells. In the case of the pre-treatment with high-LET ions, mutation frequency was around 4.0 times higher in carbon- and 1.9 times higher in helium-ion pre-treated cells than in un-pretreated cells, while no difference was observed in iron-ion pre-treated cells. On the contrary, mutation frequency in cells pre-treated with neutrons was reduced at 15% of un-pretreated cells. To identify possible mechanism(s) of the observed cellular response, cells were treated with a specific inhibitor of gap-junction mediated cell-cell communication. The results clearly showed that the observed enhancement of X-ray-induced mutation frequency in cells pre-treated with helium and carbon ions was reduced at the control level and reduction in cells pre-treated with neutrons was came back to the control level. Conclusion: There is evidence that gap-junction mediated cell-cell communication play an important role of inducing different cellular responses by pre-treatment with different radiation types. |
|||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 第二回アジア放射線連合会議(2nd Asian Congress of Radiation Research) | |||||
発表年月日 | ||||||
日付 | 2009-05-20 | |||||
日付タイプ | Issued |