WEKO3
アイテム
重粒子線に対する細胞応答におけるDNA損傷応答関連因子の機能に関する研究
https://repo.qst.go.jp/records/65835
https://repo.qst.go.jp/records/65835488095b6-7572-4163-9628-9d7445a15144
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-12-18 | |||||
| タイトル | ||||||
| タイトル | 重粒子線に対する細胞応答におけるDNA損傷応答関連因子の機能に関する研究 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
勝部, 孝則
× 勝部, 孝則× Qiang, LIU× DU, Liqing× Yan, WANG× Chang, XU× 二宮, 康晴× 王, 冰× 勝部 孝則× 二宮 康晴× 王 冰 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Artemis and XRCC4 are members of core factors for non-homologous end joining (NHEJ), a primary repair pathway of DNA double-strand breaks (DSBs). To clarify the participation of Artemis and XRCC4 in DNA damage response (DDR) to high-LET ionizing irradiation (IR), we examined the cellular responses of isogenic human cell lines deficient for these factors (Artemis-/- and XRCC4-/- cells) and a parental HCT116 cells to carbon ion-beams. Substantial increase in sensitivity of Artemis-/- and XRCC4-/- cells to carbon ion-beams as compared to HCT116 cells demonstrated participation of Artemis and XRCC4 in DDR to high-LET IR. Remarkably, the values for relative biological effectiveness (RBE) of carbon ion-beams to X-rays on killing of the Artemis-/-, XRCC4-/- and HCT116 cells appeared to be 2.7, 2.8 and 3.0, respectively. This result is inconsistent with previous reports showing that high-LET IR is much effective as compared to low-LET IR on killing of most cell lines, but not on killing of NHEJ-deficient cells including Ku70, Ku80 and DNA-PKcs mutant lines. To address this discrepancy, we have started to examine induction and repair of DSBs in the Artemis-/-, XRCC4-/- and HCT116 cells following irradiation of carbon ion-beams by gamma-H2AX nuclear foci analyses. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | H26年度HIMAC共同利用研究成果発表会 | |||||
| 発表年月日 | ||||||
| 日付 | 2015-04-21 | |||||
| 日付タイプ | Issued | |||||
