@misc{oai:repo.qst.go.jp:00065835,
 author = {勝部, 孝則 and Qiang, LIU and DU, Liqing and Yan, WANG and Chang, XU and 二宮, 康晴 and 王, 冰 and 勝部 孝則 and 二宮 康晴 and 王 冰},
 month = {Apr},
 note = {Artemis and XRCC4 are members of core factors for non-homologous end joining (NHEJ), a primary repair pathway of DNA double-strand breaks (DSBs). To clarify the participation of Artemis and XRCC4 in DNA damage response (DDR) to high-LET ionizing irradiation (IR), we examined the cellular responses of isogenic human cell lines deficient for these factors (Artemis-/- and XRCC4-/- cells) and a parental HCT116 cells to carbon ion-beams. Substantial increase in sensitivity of Artemis-/- and XRCC4-/- cells to carbon ion-beams as compared to HCT116 cells demonstrated participation of Artemis and XRCC4 in DDR to high-LET IR. Remarkably, the values for relative biological effectiveness (RBE) of carbon ion-beams to X-rays on killing of the Artemis-/-, XRCC4-/- and HCT116 cells appeared to be 2.7, 2.8 and 3.0, respectively. This result is inconsistent with previous reports showing that high-LET IR is much effective as compared to low-LET IR on killing of most cell lines, but not on killing of NHEJ-deficient cells including Ku70, Ku80 and DNA-PKcs mutant lines. To address this discrepancy, we have started to examine induction and repair of DSBs in the Artemis-/-, XRCC4-/- and HCT116 cells following irradiation of carbon ion-beams by gamma-H2AX nuclear foci analyses., H26年度HIMAC共同利用研究成果発表会},
 title = {重粒子線に対する細胞応答におけるDNA損傷応答関連因子の機能に関する研究},
 year = {2015}
}