WEKO3
アイテム
{"_buckets": {"deposit": "30e8abb9-da61-48d8-9a68-789912e954a5"}, "_deposit": {"created_by": 1, "id": "65735", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "65735"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00065735", "sets": ["29"]}, "author_link": ["647459", "647461", "647463", "647458", "647460", "647462"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2015-08-29", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To examine different effects of carbon ion beam alone or in combination with chemotherapy on putative pancreatic cancer stem cell (CSC) survival and DNA repair in comparison with X-ray, human pancreatic CSCs sorted from PANC1 and PK45 cells were treated with carbon ion beam, X-ray alone or in combination with gemcitabine. We found that the number of colony and spheroid formed from CSCs (CD44+/ESA+) after carbon ion beam was significantly reduced compared to that of X-ray, and it was extremely suppressed when combined with gemcitabine or 5-FU. The relative biological effectiveness (RBE) values for the carbon ion beam relative to X-ray at the D10 levels for CSCs were 2.23-2.66. RT Profiler PCR Array analysis showed that expressions of apoptosis-related genes (Bax, Cytochrome c), autophagy-related genes (LC3, p62), and senescence-related gene p21 were remarkably induced after carbon ion beam combined with gemcitabine compared to carbon ion beam alone or X-ray combined with gemcitabine. Not only the number but also the size of γH2AX foci in CSCs were lager 24 h after carbon ion beam combined with gemcitabine or 5-FU compared to carbon ion beam alone or X-ray combined with gemcitabine. Xenograft tumor control analysis showed that the tumors were not completely controlled even treated with 60 Gy of X-ray, but it was destroyed with 25 Gy of carbon ion beam combined with 50 mg/kg gemcitabine. In conclusion, carbon ion beam combined with gemcitabine or 5-FU has superior potential to kill pancreatic CSCs via unrepairable clustered DSB, and multiple cell death pathways.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "19th International Association of Pancreatology (IAP) Annual Meeting", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "崔, 星"}], "nameIdentifiers": [{"nameIdentifier": "647458", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Guillaume, Vares"}], "nameIdentifiers": [{"nameIdentifier": "647459", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山田, 滋"}], "nameIdentifiers": [{"nameIdentifier": "647460", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "崔 星", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "647461", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Guillaume Vares", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "647462", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山田 滋", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "647463", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS"}]}, "item_type_id": "10005", "owner": "1", "path": ["29"], "permalink_uri": "https://repo.qst.go.jp/records/65735", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-09-03"}, "publish_date": "2015-09-03", "publish_status": "0", "recid": "65735", "relation": {}, "relation_version_is_last": true, "title": ["HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS"], "weko_shared_id": -1}
HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS
https://repo.qst.go.jp/records/65735
https://repo.qst.go.jp/records/657356c31e81f-b4d1-4e17-8f25-141f0378378a
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-09-03 | |||||
タイトル | ||||||
タイトル | HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
崔, 星
× 崔, 星× Guillaume, Vares× 山田, 滋× 崔 星× Guillaume Vares× 山田 滋 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To examine different effects of carbon ion beam alone or in combination with chemotherapy on putative pancreatic cancer stem cell (CSC) survival and DNA repair in comparison with X-ray, human pancreatic CSCs sorted from PANC1 and PK45 cells were treated with carbon ion beam, X-ray alone or in combination with gemcitabine. We found that the number of colony and spheroid formed from CSCs (CD44+/ESA+) after carbon ion beam was significantly reduced compared to that of X-ray, and it was extremely suppressed when combined with gemcitabine or 5-FU. The relative biological effectiveness (RBE) values for the carbon ion beam relative to X-ray at the D10 levels for CSCs were 2.23-2.66. RT Profiler PCR Array analysis showed that expressions of apoptosis-related genes (Bax, Cytochrome c), autophagy-related genes (LC3, p62), and senescence-related gene p21 were remarkably induced after carbon ion beam combined with gemcitabine compared to carbon ion beam alone or X-ray combined with gemcitabine. Not only the number but also the size of γH2AX foci in CSCs were lager 24 h after carbon ion beam combined with gemcitabine or 5-FU compared to carbon ion beam alone or X-ray combined with gemcitabine. Xenograft tumor control analysis showed that the tumors were not completely controlled even treated with 60 Gy of X-ray, but it was destroyed with 25 Gy of carbon ion beam combined with 50 mg/kg gemcitabine. In conclusion, carbon ion beam combined with gemcitabine or 5-FU has superior potential to kill pancreatic CSCs via unrepairable clustered DSB, and multiple cell death pathways. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 19th International Association of Pancreatology (IAP) Annual Meeting | |||||
発表年月日 | ||||||
日付 | 2015-08-29 | |||||
日付タイプ | Issued |