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The ability for colony, spheroid formation, and tumorigenicity from cancer stem-like CD44+/CD24+ cells is significantly higher than that from noncancer stem-like CD44-/CD24-cells. FACS data showed that CD44+/CD24+ cells were more highly enriched after X-rays compared to carbon ion irradiation at isoeffective doses. \nThe RBE values for the carbon ion beam relative to X-ray at the D10 levels for CD44+/CD24+ cells were 2.0\u0026#8211;2.19.The number of rH2AX foci in CD44-/CD24-cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of rH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists significantly longer in carbon ion beam irradiated cells compared to X-rays.\nConclusions: Carbon ion beam has superior potential to kill pancreatic cancer stem cell-like cells, and prolonged induction of DNA damage might be one of the pivotal mechanisms of its high radiobiological effects compared to X-rays.", "subitem_description_type": "Abstract"}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1016/j.radonc.2012.08.009", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0167-8140", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Oonishi, Kazuhiko"}], "nameIdentifiers": [{"nameIdentifier": "811267", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Cui, Xing"}], "nameIdentifiers": [{"nameIdentifier": "811268", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirakawa, Hirokazu"}], "nameIdentifiers": [{"nameIdentifier": "811269", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimori, Akira"}], "nameIdentifiers": [{"nameIdentifier": "811270", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamijo, Takehiko"}], "nameIdentifiers": [{"nameIdentifier": "811271", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "811272", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokosuka, Osamu"}], "nameIdentifiers": [{"nameIdentifier": "811273", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamada, Tadashi"}], "nameIdentifiers": [{"nameIdentifier": "811274", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsujii, Hirohiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811275", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sai, Sei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811276", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirakawa, Hirokazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811277", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimori, Akira", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811278", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamijo, Takehiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811279", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Shigeru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811280", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokosuka, Osamu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811281", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamada, Tadashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811282", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/49575", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-12-13"}, "publish_date": "2012-12-13", "publish_status": "0", "recid": "49575", "relation": {}, "relation_version_is_last": true, "title": ["Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells"], "weko_shared_id": -1}
Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells
https://repo.qst.go.jp/records/49575
https://repo.qst.go.jp/records/49575edadb7ec-62aa-4365-8720-e9a0b3eacd77
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-12-13 | |||||
タイトル | ||||||
タイトル | Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Oonishi, Kazuhiko
× Oonishi, Kazuhiko× Cui, Xing× Hirakawa, Hirokazu× Fujimori, Akira× Kamijo, Takehiko× Yamada, Shigeru× Yokosuka, Osamu× Kamada, Tadashi× Tsujii, Hirohiko× Sai, Sei× Hirakawa, Hirokazu× Fujimori, Akira× Kamijo, Takehiko× Yamada, Shigeru× Yokosuka, Osamu× Kamada, Tadashi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: The effects of a carbon ion beam and X-rays on human pancreatic cancer stem-like cells were examined from the point of view of clonogenic survival and DNA repair. Materials and methods: Human pancreatic cancer stem-like cells were treated with and without carbon ion and X-ray irradiation, and then colony, spheroid and tumor formation assays as well as rH2AX foci formation assay were performed. Results: The relative biological effectiveness (RBE) values of a carbon ion beam relative to X-ray for the MIA PaCa-2 and BxPc-3 cells at the D10 values were 1.85–2.10. The ability for colony, spheroid formation, and tumorigenicity from cancer stem-like CD44+/CD24+ cells is significantly higher than that from noncancer stem-like CD44-/CD24-cells. FACS data showed that CD44+/CD24+ cells were more highly enriched after X-rays compared to carbon ion irradiation at isoeffective doses. The RBE values for the carbon ion beam relative to X-ray at the D10 levels for CD44+/CD24+ cells were 2.0–2.19.The number of rH2AX foci in CD44-/CD24-cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of rH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists significantly longer in carbon ion beam irradiated cells compared to X-rays. Conclusions: Carbon ion beam has superior potential to kill pancreatic cancer stem cell-like cells, and prolonged induction of DNA damage might be one of the pivotal mechanisms of its high radiobiological effects compared to X-rays. |
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書誌情報 |
Radiotherapy and Oncology 巻 105, 号 2, p. 258-265, 発行日 2012-09 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0167-8140 | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.1016/j.radonc.2012.08.009 |