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The levels of GTP-bound Rac1 and RhoA were determined by the active GTPase pull-down assay with or without a proteasome inhibitor, and the binding of E3 ubiquitin ligase to GTP-bound Rac1 was examined by immunoprecipitation.\n\\nResults\nC-ion irradiation reduced the levels of GTP-bound Rac1 and RhoA, two major regulators of cell motility, in MIAPaCa-2 cells and GTP-bound Rac1 in AsPC-1 and BxPC-3 cells. Proteasome inhibition reversed the effect, indicating that C-ion irradiation induced Rac1 and RhoA degradation via the ubiquitin (Ub)-proteasome pathway. E3 Ub ligase X-linked inhibitor of apoptosis protein (XIAP) which directly targets Rac1, was selectively induced in C-ion irradiated MIAPaCa-2 cells and co-precipitated with GTP-bound Rac1 in C-ion-irradiated cells, which was associated with Rac1 ubiquitination. Cell migration and invasion reduced by C-ion radiation were restored by siRNA-mediated XIAP knockdown, indicating that XIAP is involved in C-ion-induced inhibition of cell motility.\n\\nConclusion\nIn contrast to X-ray irradiation, C-ion treatment inhibited the activity of Rac1 and RhoA in MIAPaCa-2 cells and Rac1 in AsPC-1 and BxPC-3 cells via Ub-mediated proteasomal degradation, thereby blocking the motility of these pancreatic cancer cells.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Tarrytown Ny : Elsevier Science Inc"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "doi:10.1016/j.ijrobp.2015.05.009", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0360-3016", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fujita, Mayumi"}], "nameIdentifiers": [{"nameIdentifier": "474614", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imadome, Kaori"}], "nameIdentifiers": [{"nameIdentifier": "474615", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shoji, Yoshimi"}], "nameIdentifiers": [{"nameIdentifier": "474616", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Isozaki, Teturou"}], "nameIdentifiers": [{"nameIdentifier": "474617", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Endo, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "474618", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "474619", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imai, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "474620", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "藤田 真由美", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474621", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "今留 香織", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474622", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "荘司 好美", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474623", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "磯崎 哲朗", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474624", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "遠藤 悟史", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474625", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山田 滋", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474626", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "今井 高志", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "474627", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation ", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation "}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/47402", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-05-18"}, "publish_date": "2016-05-18", "publish_status": "0", "recid": "47402", "relation": {}, "relation_version_is_last": true, "title": ["Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation "], "weko_shared_id": -1}
Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation
https://repo.qst.go.jp/records/47402
https://repo.qst.go.jp/records/47402637cfc3c-201b-4ad4-8d43-47db2f97f79f
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-05-18 | |||||
タイトル | ||||||
タイトル | Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Fujita, Mayumi
× Fujita, Mayumi× Imadome, Kaori× Shoji, Yoshimi× Isozaki, Teturou× Endo, Satoshi× Yamada, Shigeru× Imai, Takashi× 藤田 真由美× 今留 香織× 荘司 好美× 磯崎 哲朗× 遠藤 悟史× 山田 滋× 今井 高志 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose This study investigated the mechanisms underlying the inhibition of cancer cell migration and invasion by carbon (C)-ion irradiation. \nMethods and Materials Human pancreatic cancer cells MIAPaCa-2, AsPC-1, and BxPC-3 were treated by X-ray (4 Gy) or C-ion (0.5, 1, 2, or 4 Gy) irradiation, and their migration and invasion were assessed 2 days later. The levels of GTP-bound Rac1 and RhoA were determined by the active GTPase pull-down assay with or without a proteasome inhibitor, and the binding of E3 ubiquitin ligase to GTP-bound Rac1 was examined by immunoprecipitation. \nResults C-ion irradiation reduced the levels of GTP-bound Rac1 and RhoA, two major regulators of cell motility, in MIAPaCa-2 cells and GTP-bound Rac1 in AsPC-1 and BxPC-3 cells. Proteasome inhibition reversed the effect, indicating that C-ion irradiation induced Rac1 and RhoA degradation via the ubiquitin (Ub)-proteasome pathway. E3 Ub ligase X-linked inhibitor of apoptosis protein (XIAP) which directly targets Rac1, was selectively induced in C-ion irradiated MIAPaCa-2 cells and co-precipitated with GTP-bound Rac1 in C-ion-irradiated cells, which was associated with Rac1 ubiquitination. Cell migration and invasion reduced by C-ion radiation were restored by siRNA-mediated XIAP knockdown, indicating that XIAP is involved in C-ion-induced inhibition of cell motility. \nConclusion In contrast to X-ray irradiation, C-ion treatment inhibited the activity of Rac1 and RhoA in MIAPaCa-2 cells and Rac1 in AsPC-1 and BxPC-3 cells via Ub-mediated proteasomal degradation, thereby blocking the motility of these pancreatic cancer cells. |
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書誌情報 |
International Journal of Radiation Oncology Biology Physics 巻 93, 号 1, p. 173-180, 発行日 2015-05 |
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出版者 | ||||||
出版者 | Tarrytown Ny : Elsevier Science Inc | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0360-3016 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | doi:10.1016/j.ijrobp.2015.05.009 |