WEKO3
アイテム
A simultaneous space sampling method for DNA fraction collection using a comb structure in microfluidic devices.
https://repo.qst.go.jp/records/46976
https://repo.qst.go.jp/records/46976722cf05e-80e2-46f0-bf64-049b1a12e5b5
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-12-01 | |||||
| タイトル | ||||||
| タイトル | A simultaneous space sampling method for DNA fraction collection using a comb structure in microfluidic devices. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Li, Zheyu
× Li, Zheyu× Sun, Kai× Sunayama, Misato× Araki, Ryoko× Ueno, Kosei× Abe, Masumi× Misawa, Hiroaki× 砂山 美里× 荒木 良子× 安倍 真澄 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fraction collection of selected components from a complex mixture plays a critical role in biomedical research, environmental analysis, and biotechnology. Here, we introduce a novel electrophoretic chip device based on a signal processing theorem that allows simultaneous space sampling for fractionation of ssDNA target fragments. Ten parallel extraction channels, which covered 1.5-mm-long sampling ranges, were used to facilitate the capturing of fast-moving fragments. Furthermore, the space sampling extraction made it possible to acquire pure collection, even from partly overlapping fragments that had been insufficiently separated after a short electrophoretic run. Fragments of 180, 181, and 182 bases were simultaneously collected, and then the recovered DNA was PCR amplified and assessed by CE analysis. The 181-base target was shown to be isolated in a 70-mm-long separation length within 10 min, in contrast to the >50 min required for the 300-mm-long separation channel in our previous study. This method provides effective combination of time and space, which is a breakthrough in the traditional concept of fraction collection on a chip. | |||||
| 書誌情報 |
Electrophoresis 巻 32, 号 23, p. 3392-3398, 発行日 2011-11 |
|||||
| 出版者 | ||||||
| 出版者 | Wiley-VCH | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0173-0835 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 22072547 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/elps.201100362 | |||||
