@article{oai:repo.qst.go.jp:00046976,
 author = {Li, Zheyu and Sun, Kai and Sunayama, Misato and Araki, Ryoko and Ueno, Kosei and Abe, Masumi and Misawa, Hiroaki and 砂山 美里 and 荒木 良子 and 安倍 真澄},
 issue = {23},
 journal = {Electrophoresis},
 month = {Nov},
 note = {Fraction collection of selected components from a complex mixture plays a critical role in biomedical research, environmental analysis, and biotechnology. Here, we introduce a novel electrophoretic chip device based on a signal processing theorem that allows simultaneous space sampling for fractionation of ssDNA target fragments. Ten parallel extraction channels, which covered 1.5-mm-long sampling ranges, were used to facilitate the capturing of fast-moving fragments. Furthermore, the space sampling extraction made it possible to acquire pure collection, even from partly overlapping fragments that had been insufficiently separated after a short electrophoretic run. Fragments of 180, 181, and 182 bases were simultaneously collected, and then the recovered DNA was PCR amplified and assessed by CE analysis. The 181-base target was shown to be isolated in a 70-mm-long separation length within 10 min, in contrast to the >50 min required for the 300-mm-long separation channel in our previous study. This method provides effective combination of time and space, which is a breakthrough in the traditional concept of fraction collection on a chip.},
 pages = {3392--3398},
 title = {A simultaneous space sampling method for DNA fraction collection using a comb structure in microfluidic devices.},
 volume = {32},
 year = {2011}
}