WEKO3
アイテム
{"_buckets": {"deposit": "df4f1e34-17a5-4978-a8df-fd520807116c"}, "_deposit": {"created_by": 1, "id": "46732", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "46732"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00046732", "sets": ["1"]}, "author_link": ["466002", "466000", "466003", "465994", "465995", "465999", "466001", "466004", "466005", "466008", "465997", "465998", "466007", "466006", "465996"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "3224", "bibliographicPageStart": "3216", "bibliographicVolumeNumber": "73", "bibliographic_titles": [{"bibliographic_title": "Cancer Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "For tumor radiotherapy, the in vivo detection of early cellular responses is important for predicting therapeutic efficacy. Mn2+ is used as a positive contrast agent in manganese-enhanced MRI (MEMRI) and is expected to behave as a mimic of Ca2+ in many biologic systems. We conducted in vitro and in vivo MRI experiments with Mn2+ to investigate whether MEMRI can be used to detect cell alterations as an early-phase tumor response after radiotherapy. Colon-26 cells or a subcutaneously grafted colon-26 tumor model were irradiated with 20 Gy of X-rays. One day after irradiation, a significant augmentation of G2\u0026#8211;M-phase cells, indicating a cell-cycle arrest, was observed in the irradiated cells in comparison with the control cells, although both early and late apoptotic alterations were rarely observed. The MEMRI signal in radiation-exposed tumor cells (R1: 0.77 +- 0.01 s\u0026#8722;1) was significantly lower than that in control cells (R1: 0.82 +- 0.01 s\u0026#8722;1) in vitro. MEMRI signal reduction was also observed in the in vivo tumor model 24 hours after irradiation (R1 of radiation: 0.97 +- 0.02 s\u0026#8722;1, control: 1.10 +- 0.02 s\u0026#8722;1), along with cell-cycle and proliferation alterations identified with immunostaining (cyclin D1 and Ki-67). Therefore, MEMRI after tumor radiotherapy was successfully used to detect cell alterations as an early-phase cellular response in vitro and in vivo.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1158/0008-5472.can-12-3837", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0008-5472", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Saito, Shigeyoshi"}], "nameIdentifiers": [{"nameIdentifier": "465994", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasegawa, Sumitaka"}], "nameIdentifiers": [{"nameIdentifier": "465995", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekita, Aiko"}], "nameIdentifiers": [{"nameIdentifier": "465996", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bakalova-Zheleva, Rumiana"}], "nameIdentifiers": [{"nameIdentifier": "465997", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Takako"}], "nameIdentifiers": [{"nameIdentifier": "465998", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murase, Kenya"}], "nameIdentifiers": [{"nameIdentifier": "465999", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saga, Tsuneo"}], "nameIdentifiers": [{"nameIdentifier": "466000", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aoki, Ichio"}], "nameIdentifiers": [{"nameIdentifier": "466001", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "齋藤 茂芳", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466002", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "長谷川 純崇", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466003", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "バカロバ ルミアナ", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466004", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古川 高子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466005", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "村瀬 研也", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466006", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "佐賀 恒夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466007", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "青木 伊知男", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466008", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo."}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/46732", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-03-05"}, "publish_date": "2014-03-05", "publish_status": "0", "recid": "46732", "relation": {}, "relation_version_is_last": true, "title": ["Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo."], "weko_shared_id": -1}
Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo.
https://repo.qst.go.jp/records/46732
https://repo.qst.go.jp/records/46732be28bcd2-cb96-4bfa-a7b2-65cd2dc77876
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-03-05 | |||||
タイトル | ||||||
タイトル | Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Saito, Shigeyoshi
× Saito, Shigeyoshi× Hasegawa, Sumitaka× Sekita, Aiko× Bakalova-Zheleva, Rumiana× Furukawa, Takako× Murase, Kenya× Saga, Tsuneo× Aoki, Ichio× 齋藤 茂芳× 長谷川 純崇× バカロバ ルミアナ× 古川 高子× 村瀬 研也× 佐賀 恒夫× 青木 伊知男 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For tumor radiotherapy, the in vivo detection of early cellular responses is important for predicting therapeutic efficacy. Mn2+ is used as a positive contrast agent in manganese-enhanced MRI (MEMRI) and is expected to behave as a mimic of Ca2+ in many biologic systems. We conducted in vitro and in vivo MRI experiments with Mn2+ to investigate whether MEMRI can be used to detect cell alterations as an early-phase tumor response after radiotherapy. Colon-26 cells or a subcutaneously grafted colon-26 tumor model were irradiated with 20 Gy of X-rays. One day after irradiation, a significant augmentation of G2–M-phase cells, indicating a cell-cycle arrest, was observed in the irradiated cells in comparison with the control cells, although both early and late apoptotic alterations were rarely observed. The MEMRI signal in radiation-exposed tumor cells (R1: 0.77 +- 0.01 s−1) was significantly lower than that in control cells (R1: 0.82 +- 0.01 s−1) in vitro. MEMRI signal reduction was also observed in the in vivo tumor model 24 hours after irradiation (R1 of radiation: 0.97 +- 0.02 s−1, control: 1.10 +- 0.02 s−1), along with cell-cycle and proliferation alterations identified with immunostaining (cyclin D1 and Ki-67). Therefore, MEMRI after tumor radiotherapy was successfully used to detect cell alterations as an early-phase cellular response in vitro and in vivo. | |||||
書誌情報 |
Cancer Research 巻 73, 号 11, p. 3216-3224, 発行日 2013-05 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0008-5472 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1158/0008-5472.can-12-3837 |