WEKO3
アイテム
{"_buckets": {"deposit": "55026659-c630-4642-af12-d1abd7a522a7"}, "_deposit": {"created_by": 1, "id": "46113", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "46113"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00046113", "sets": ["1"]}, "author_link": ["459079", "459082", "459088", "459086", "459085", "459087", "459089", "459084", "459090", "459083", "459080", "459078", "459081"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "24", "bibliographicPageEnd": "4284", "bibliographicPageStart": "4277", "bibliographicVolumeNumber": "30", "bibliographic_titles": [{"bibliographic_title": "Electrophoresis"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "For the fractionation of fragments of interest from selective PCR products generated by high coverage gene expression profiling (HiCEP) analysis, high-resolution with the ability to discriminate and fractionate fragments differing by one base (base pair) in size is highly required. We report here on a new 4-inch diameter spiral-channel chip device for automatic high-fidelity fractionation. Overlapping DNA fragments of 180, 181 and 182 bases, with only one-base difference in size, were successfully fractionated. The collected fragments were PCR amplified, and then evaluated by size checking analysis, DNA sequencing, and homolog search. The high-resolution fractionation has been achieved because of the combined contributions of (i) the high-resolution separation using a 30 cm long spiral channel, (ii) a blocking technique to avoid contamination from unselected fragments during CE, and (iii) precise micro-scale target extraction. Contaminations due to unselected fractions have been greatly decreased to a negligible level by optimization of the extraction position and extraction time corresponding to the targeted segment only. This technique can be adapted to a wide range of applications, such as protein or cell collections where requirements for the high purity are more important than the amount of recovered fractionated material.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0173-0835", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Son, Kai"}], "nameIdentifiers": [{"nameIdentifier": "459078", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Nobuko"}], "nameIdentifiers": [{"nameIdentifier": "459079", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Li, Zheyu"}], "nameIdentifiers": [{"nameIdentifier": "459080", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Araki, Ryoko"}], "nameIdentifiers": [{"nameIdentifier": "459081", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueno, Kousei"}], "nameIdentifiers": [{"nameIdentifier": "459082", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Juodkazis, Saulius"}], "nameIdentifiers": [{"nameIdentifier": "459083", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Abe, Masumi"}], "nameIdentifiers": [{"nameIdentifier": "459084", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Noji, Sumihare"}], "nameIdentifiers": [{"nameIdentifier": "459085", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Misawa, Hiroaki"}], "nameIdentifiers": [{"nameIdentifier": "459086", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鈴木 展子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "459087", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "荒木 良子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "459088", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "上野 貢生", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "459089", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安倍 真澄", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "459090", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip."}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/46113", "pubdate": {"attribute_name": "公開日", "attribute_value": "2011-06-20"}, "publish_date": "2011-06-20", "publish_status": "0", "recid": "46113", "relation": {}, "relation_version_is_last": true, "title": ["High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip."], "weko_shared_id": -1}
High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip.
https://repo.qst.go.jp/records/46113
https://repo.qst.go.jp/records/4611373531d10-793a-4990-816c-d56556104fcf
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2011-06-20 | |||||
タイトル | ||||||
タイトル | High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Son, Kai
× Son, Kai× Suzuki, Nobuko× Li, Zheyu× Araki, Ryoko× Ueno, Kousei× Juodkazis, Saulius× Abe, Masumi× Noji, Sumihare× Misawa, Hiroaki× 鈴木 展子× 荒木 良子× 上野 貢生× 安倍 真澄 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For the fractionation of fragments of interest from selective PCR products generated by high coverage gene expression profiling (HiCEP) analysis, high-resolution with the ability to discriminate and fractionate fragments differing by one base (base pair) in size is highly required. We report here on a new 4-inch diameter spiral-channel chip device for automatic high-fidelity fractionation. Overlapping DNA fragments of 180, 181 and 182 bases, with only one-base difference in size, were successfully fractionated. The collected fragments were PCR amplified, and then evaluated by size checking analysis, DNA sequencing, and homolog search. The high-resolution fractionation has been achieved because of the combined contributions of (i) the high-resolution separation using a 30 cm long spiral channel, (ii) a blocking technique to avoid contamination from unselected fragments during CE, and (iii) precise micro-scale target extraction. Contaminations due to unselected fractions have been greatly decreased to a negligible level by optimization of the extraction position and extraction time corresponding to the targeted segment only. This technique can be adapted to a wide range of applications, such as protein or cell collections where requirements for the high purity are more important than the amount of recovered fractionated material. | |||||
書誌情報 |
Electrophoresis 巻 30, 号 24, p. 4277-4284, 発行日 2009-12 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0173-0835 |