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Development of Positron Emission Tomography Imaging by 64Cu-labeled Fab for Detecting ERC/mesothelin in a Mesothelioma Mouse Model
https://repo.qst.go.jp/records/45844
https://repo.qst.go.jp/records/45844ed5033dd-6182-4757-9ea0-06f1905531c4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-12-11 | |||||
| タイトル | ||||||
| タイトル | Development of Positron Emission Tomography Imaging by 64Cu-labeled Fab for Detecting ERC/mesothelin in a Mesothelioma Mouse Model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshida, Chisato
× Yoshida, Chisato× Sogawa, Chizuru× Tsuji, Atsushi× Sudou, Hitomi× Sugyou, Aya× Uehara, Tomoya× Hino, Okio× Yoshii, Yukie× Fujibayashi, Yasuhisa× Fukumura, Toshimitsu× Koizumi, Mitsuru× Arano, Yasushi× Saga, Tsuneo× Yoshida, Chisato× Sogawa, Chizuru× Tsuji, Atsushi× Sudo, Hitomi× Sugyo, Aya× Uehara, Tomoya× Yoshii, Yukie× Fukumura, Toshimitsu× Koizumi, Mitsuru× Arano, Yasushi× Saga, Tsuneo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background Malignant mesothelioma is a highly aggressive form of cancer. Curative surgery is the only effective therapy for mesothelioma, and therefore early diagnosis is important. However, early diagnosis is difficult using current diagnostic imaging techniques, and a new imaging method for early diagnosis is urgently required. We evaluated the affinity of radiolabeled monoclonal antibodies to the C-terminal fragment of ERC/mesothelin for this purpose. Methods 111 In-labeled or125 I-labeled IgG against C-terminal fragment of ERC and its Fab fragment were evaluated in vitro by cell binding, competitive inhibition, and cellular internalization assays, and in vivo by biodistribution in mice bearing ERC-expressing tumors. Next, the Fab fragment was labeled with the positron emitte 64 Cu and evaluated by positron emission tomography (PET). Results Radiolabeled IgG and Fab showed specific binding to ERC-expressing mesothelioma cells with high affinity. Both radiolabeled IgG and Fab internalized into cells after binding to ERC on the cell surface. 111 In-labeled IgG accumulated in ERC-expressing tumors and resulted in a moderate tumor-to-blood ratio at 4 days after injection. Furthermore, PET using 64 Cu-labeled Fab visualized the tumor at 6 h after injection. Conclusion64Cu-labeled Fab can be useful for ERC-specific PET imaging, and can thus facilitate improved diagnosis of patients with early-stage mesothelioma. Nucl Med Commun 00:000–000 c 2010 Wolters Kluwer Health |Lippincott Williams & Wilkins. |
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| 書誌情報 |
Nuclear Medicine Communications 巻 31, 号 5, p. 380-388, 発行日 2010-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0143-3636 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1097/mnm.0b013e328336733d | |||||
