@article{oai:repo.qst.go.jp:00045844,
 author = {Yoshida, Chisato and Sogawa, Chizuru and Tsuji, Atsushi and Sudou, Hitomi and Sugyou, Aya and Uehara, Tomoya and Hino, Okio and Yoshii, Yukie and Fujibayashi, Yasuhisa and Fukumura, Toshimitsu and Koizumi, Mitsuru and Arano, Yasushi and Saga, Tsuneo and Yoshida, Chisato and Sogawa, Chizuru and Tsuji, Atsushi and Sudo, Hitomi and Sugyo, Aya and Uehara, Tomoya and Yoshii, Yukie and Fukumura, Toshimitsu and Koizumi, Mitsuru and Arano, Yasushi and Saga, Tsuneo},
 issue = {5},
 journal = {Nuclear Medicine Communications},
 month = {Jan},
 note = {Background  Malignant mesothelioma is a highly
aggressive form of cancer. Curative surgery is the only
effective therapy for mesothelioma, and therefore early diagnosis is important. 
However, early diagnosis is difficult using current diagnostic imaging techniques, and a new imaging method for early diagnosis is urgently required.
We evaluated the affinity of radiolabeled monoclonal antibodies to the C-terminal fragment of ERC/mesothelin for this purpose.
Methods 111 In-labeled or125 I-labeled IgG against C-terminal fragment of ERC and its Fab fragment were evaluated in vitro by cell binding, competitive inhibition, and cellular internalization assays, and in vivo by biodistribution in mice bearing ERC-expressing tumors. Next, the Fab fragment was labeled with the positron emitte 64 Cu and evaluated by positron emission tomography (PET).
Results  Radiolabeled IgG and Fab showed specific binding to ERC-expressing mesothelioma cells with high affinity. Both radiolabeled IgG and Fab internalized into
cells after binding to ERC on the cell surface.
111 In-labeled IgG accumulated in ERC-expressing tumors and resulted in a moderate tumor-to-blood ratio at 4 days after injection. Furthermore, PET using 64 Cu-labeled Fab visualized the tumor at 6 h after injection.
Conclusion64Cu-labeled Fab can be useful for ERC-specific PET imaging, and can thus facilitate improved diagnosis of patients with early-stage mesothelioma.  
Nucl Med Commun 00:000–000  c   2010 Wolters Kluwer Health |Lippincott Williams & Wilkins.},
 pages = {380--388},
 title = {Development of Positron Emission Tomography Imaging by 64Cu-labeled Fab for Detecting ERC/mesothelin in a Mesothelioma Mouse Model},
 volume = {31},
 year = {2010}
}