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A Fast, Simple Method for Screening Radiation Susceptibility Genes by RNA Interference
https://repo.qst.go.jp/records/43718
https://repo.qst.go.jp/records/437187fd5e316-9c3a-487a-9f2f-6efcda21756a
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2005-07-07 | |||||
タイトル | ||||||
タイトル | A Fast, Simple Method for Screening Radiation Susceptibility Genes by RNA Interference | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Tsuji, Atsushi
× Tsuji, Atsushi× Sudou, Hitomi× Sugyou, Aya× Ohtuki, Marika× Miyagishi, Makoto× Taira, Kazunari× Imai, Takashi× Harada, Yoshinobu× 辻 厚至× 須藤 仁美× 須尭 綾× 大月 まり香× 今井 高志× 原田 良信 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes. | |||||
書誌情報 |
Biochemical and Biophysical Research Communications 巻 333, p. 1370-1377, 発行日 2005-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0006-291X |