@article{oai:repo.qst.go.jp:00043718,
 author = {Tsuji, Atsushi and Sudou, Hitomi and Sugyou, Aya and Ohtuki, Marika and Miyagishi, Makoto and Taira, Kazunari and Imai, Takashi and Harada, Yoshinobu and 辻 厚至 and 須藤 仁美 and 須尭 綾 and 大月 まり香 and 今井 高志 and 原田 良信},
 journal = {Biochemical and Biophysical Research Communications},
 month = {Aug},
 note = {Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes.},
 pages = {1370--1377},
 title = {A Fast, Simple Method for Screening Radiation Susceptibility Genes by RNA Interference},
 volume = {333},
 year = {2005}
}