{"created":"2023-05-15T14:33:42.124055+00:00","id":43718,"links":{},"metadata":{"_buckets":{"deposit":"f28313d7-61d5-4052-92d7-a37a6de6847b"},"_deposit":{"created_by":1,"id":"43718","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43718"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043718","sets":["1"]},"author_link":["434682","434681","434675","434678","434674","434676","434684","434687","434680","434677","434679","434685","434686","434683"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1377","bibliographicPageStart":"1370","bibliographicVolumeNumber":"333","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tsuji, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"434674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sudou, Hitomi"}],"nameIdentifiers":[{"nameIdentifier":"434675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugyou, Aya"}],"nameIdentifiers":[{"nameIdentifier":"434676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohtuki, Marika"}],"nameIdentifiers":[{"nameIdentifier":"434677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyagishi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"434678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taira, Kazunari"}],"nameIdentifiers":[{"nameIdentifier":"434679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"434680","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Harada, Yoshinobu"}],"nameIdentifiers":[{"nameIdentifier":"434681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須藤 仁美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須尭 綾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大月 まり香","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田 良信","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434687","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Fast, Simple Method for Screening Radiation Susceptibility Genes by RNA Interference","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Fast, Simple Method for Screening Radiation Susceptibility Genes by RNA Interference"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-07-07"},"publish_date":"2005-07-07","publish_status":"0","recid":"43718","relation_version_is_last":true,"title":["A Fast, Simple Method for Screening Radiation Susceptibility Genes by RNA Interference"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:23:54.661010+00:00"}