WEKO3
アイテム
{"_buckets": {"deposit": "70537e67-3b2c-475e-b139-edad62b0f126"}, "_deposit": {"created_by": 1, "id": "43469", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "43469"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00043469", "sets": ["1"]}, "author_link": ["432263", "432262", "432264", "432266", "432273", "432269", "432268", "432265", "432270", "432272", "432261", "432271", "432267"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2001-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "602", "bibliographicPageStart": "598", "bibliographicVolumeNumber": "156", "bibliographic_titles": [{"bibliographic_title": "Radiation Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We reported previously that exposure of normal human fibroblasts in G2 phase of the cell cycle to high-LET radiation produces a much higher frequency of isochromatid breaks than exposure to gamma rays. We concluded that an increase in the production of isochromatid breaks is a signature of initial high-LET radiation-induced G2-phase damage. In this paper, we report the repair kinetics of isochromatid breaks induced by high-LET radiation in normal G2-phase human fibroblasts. Exponentially growing human fibroblasts (AG1522) were irradiated with gamma rays or energetic carbon (290 MeV/nucleon), silicon (490 MeV/nucleon), or iron (200 MeV/nucleon) ions. Prematurely condensed chromosomes were induced by calyculin A after different postirradiation incubation times ranging from 0 to 600 min. Chromosomes were stained with Giemsa, and aberrations were scored in cells at G2 phase. G2-phase fragments, the result of the induction of isochromatid breaks, decreased quickly with incubation time. The curve for the kinetics of the rejoining of chromatid-type breaks showed a slight upward curvature with time after exposure to 440 keV/microm iron particles, probably due to isochromatid-isochromatid break rejoining. The formation of chromatid exchanges after exposure to high-LET radiation therefore appears to be underestimated, because isochromatid-isochromatid exchanges cannot be detected. Increased induction of isochromatid breaks and rejoining of isochromatid breaks affect the overall kinetics of chromatid-type break rejoining after exposure to high-LET radiation.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0033-7587", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kawata, Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "432261", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Durante, Marco"}], "nameIdentifiers": [{"nameIdentifier": "432262", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furusawa, Yoshiya"}], "nameIdentifiers": [{"nameIdentifier": "432263", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "George, Kerry"}], "nameIdentifiers": [{"nameIdentifier": "432264", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Hisao"}], "nameIdentifiers": [{"nameIdentifier": "432265", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wu, Honglu"}], "nameIdentifiers": [{"nameIdentifier": "432266", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "A, Cucinotta Francesca"}], "nameIdentifiers": [{"nameIdentifier": "432267", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "川田 哲也", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "432268", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古澤 佳也", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "432269", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ジョージ ケリー", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "432270", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "伊東 久夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "432271", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ウー ホングル", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "432272", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "クシノッタ フランク", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "432273", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts."}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/43469", "pubdate": {"attribute_name": "公開日", "attribute_value": "2004-08-26"}, "publish_date": "2004-08-26", "publish_status": "0", "recid": "43469", "relation": {}, "relation_version_is_last": true, "title": ["Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts."], "weko_shared_id": -1}
Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts.
https://repo.qst.go.jp/records/43469
https://repo.qst.go.jp/records/43469b0dc8c9e-1276-44da-bac4-9c27b1ccbad0
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2004-08-26 | |||||
タイトル | ||||||
タイトル | Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Kawata, Tetsuya
× Kawata, Tetsuya× Durante, Marco× Furusawa, Yoshiya× George, Kerry× Ito, Hisao× Wu, Honglu× A, Cucinotta Francesca× 川田 哲也× 古澤 佳也× ジョージ ケリー× 伊東 久夫× ウー ホングル× クシノッタ フランク |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We reported previously that exposure of normal human fibroblasts in G2 phase of the cell cycle to high-LET radiation produces a much higher frequency of isochromatid breaks than exposure to gamma rays. We concluded that an increase in the production of isochromatid breaks is a signature of initial high-LET radiation-induced G2-phase damage. In this paper, we report the repair kinetics of isochromatid breaks induced by high-LET radiation in normal G2-phase human fibroblasts. Exponentially growing human fibroblasts (AG1522) were irradiated with gamma rays or energetic carbon (290 MeV/nucleon), silicon (490 MeV/nucleon), or iron (200 MeV/nucleon) ions. Prematurely condensed chromosomes were induced by calyculin A after different postirradiation incubation times ranging from 0 to 600 min. Chromosomes were stained with Giemsa, and aberrations were scored in cells at G2 phase. G2-phase fragments, the result of the induction of isochromatid breaks, decreased quickly with incubation time. The curve for the kinetics of the rejoining of chromatid-type breaks showed a slight upward curvature with time after exposure to 440 keV/microm iron particles, probably due to isochromatid-isochromatid break rejoining. The formation of chromatid exchanges after exposure to high-LET radiation therefore appears to be underestimated, because isochromatid-isochromatid exchanges cannot be detected. Increased induction of isochromatid breaks and rejoining of isochromatid breaks affect the overall kinetics of chromatid-type break rejoining after exposure to high-LET radiation. | |||||
書誌情報 |
Radiation Research 巻 156, 号 5, p. 598-602, 発行日 2001-11 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0033-7587 |