量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Background/Purpose: We have successfully visualized amyloid- (A) deposition in a mouse model of Alzheimer’s disease (AD) with the aid of newly developed amyloid imaging tracer 123/125I-DRM106, and suggested different binding site in aggregated A for this compound with popularly used amyloid imaging tracers such as PiB (Pittsburgh Compound B) in our previous study (J Nucl Med; 2015). In the present study, we have investigated the autoradiogram of radioiodinated DRM106 and other several amyloid imaging tracers in postmortem AD brain to examine the possibility of distinguishing different types of A deposits by combination of these tracers.
Materials & Methods: The AD brain sections containing the hippocampal and parahippocampal regions were incubated with radioactive tracers (11C-PiB; 5nM, 3H-AZD2184; 5nM, 125I-DRM106; 5nM) for 60 minutes, followed by consequent processes of autoradiographic analysis. Amyloid deposition was determined by fluorescent counterstaining with FSB and immunohistochemical staining with anti-A antibody (6E10).
Results: Autoradiographic images showed there is greatly difference in the feature of binding to brain subregions between tested tracers. There were greatly abundant binding sites for 11C-PiB and 3H-AZD2184, but not for 125I-DRM106, in the presubiculum of AD brain, where, abundant super-diffuse A deposits reacted with 6E10, was FSB-negative by fluorescent microscopic observation. These data indicate preferentiality of DRM106 to highly maturated A deposits compared with PiB and AZD2184.
Conclusions: The present result reveal possible contribution of those minor A pathology, such as super-diffuse plaques, to binding sites for these popularly used tracers, and it might be distinguishable by combination scans with different type amyloid imaging tracers such as PiB/AZD2184 and DRM106.
会議概要（会議名, 開催地, 会期, 主催者等）
Japan-China Nuclear Medicine joint symposium in Okinawa
Radiolabelling of Super-diffuse Amyloid-b Deposition with Popularly Used Amyloid Imaging Ligands in Postmortem Brain of Alzheimer’s Disease
