量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Using cultured and nude mouse tumor cells (IA) derived from a human lung cancer, we had previously reported the early and rapid survival recovery (PLD repair) occurring after irradiation. There are only a few studies in the molecular biological level and gene expression in association with PLD repair, and on limiting its effect after carbon-ion beam irradiation to cancer cells in vivo, no study was reported. In this study we tried to detect the mechanism of DNA damage and repair of the IA tumor cell after carbon-ion beam or X-ray irradiation. At first, colony assay method was performed to compare the time dependent cell survival of the IA cells after each irradiation. Second, to search the genes causing PLD repair after each irradiation, we evaluated the time dependent gene expression by using semi-quantitative RT-PCR with the selected 33 genes reported to be related to DNA repair. From the result of survival assay, PLD repair was found only after irradiation of X-ray in this IA cells, whereas it was inhibited after carbon-ion beam irradiation. Genes showing significantly higher expressions after X-ray irradiation than those after carbon-ion beam irradiation were 5 genes and lower expressions after X-ray irradiation than those after carbon-ion beam irradiation were 7 genes. We think these 12 genes are the candidate ones associated with PLD repair among the selected 33 DNA repair related genes.
重粒子線治療における肺癌腫瘍の至適分割法の研究：低酸素下加速分裂細胞の放射線感受性と
