量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Multicentric chromosomes, as represented by dicentric chromosomes, are
considered to be sensitive and specific biomarkers for assessing radiation
dose. Efforts have been made to precisely localize centromeres by
fluorescence in situ hybridization (FISH) using pan-centromeric peptide
nucleic acid (PNA) probes, which bind to the target centromeric DNA
sequences quickly. We analyzed radiation-induced chromosomal aberrations by
our modified technique that is simpler than the standard PNA - FISH
protocol. Under our experimental conditions, the detection efficiency of
centromeric signals was 100%, while that of telomeric signals was 90-100%.
Unequivocal detection of radiation-induced multicentric chromosomes by fluorescence in situ hybridization using peptide nucleic acid probes
