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Disaccharide-tag for highly sensitive identification of O-GlcNAc-modified proteins in mammalian cells.
https://repo.qst.go.jp/records/86175
https://repo.qst.go.jp/records/86175a0cb6ac0-1054-4fb5-af50-24f69a0b85f9
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-05-24 | |||||
| タイトル | ||||||
| タイトル | Disaccharide-tag for highly sensitive identification of O-GlcNAc-modified proteins in mammalian cells. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Abo, Hirohito
× Abo, Hirohito× Kume, Masahiko× Federico Pecori× Taichi, Miura× Naoki Matsumoto× Shoko, Nishihara× Kazuo Yamamoto× Taichi, Miura× Shoko, Nishihara |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | O-GlcNAcylation is the only sugar modification for proteins present in the cytoplasm and nucleus and is thought to be involved in the regulation of protein function and localization. Currently, several methods are known for detecting O-GlcNAcylated proteins using monoclonal antibodies or wheat germ agglutinin, but these methods have some limitations in their sensitivity and quantitative comparison. We developed a new disaccharide-tag method to overcome these problems. This is a method in which a soluble GalNAc transferase is expressed intracellularly, extended to a disaccharide of GalNAc-GlcNAc, and detected using a Wisteria japonica agglutinin specific to this disaccharide. We verified the method using human c-Rel protein and also highly sensitively compared the difference in O-GlcNAc modification of intracellular proteins associated with differentiation from embryonic stem cell (ESC) to epiblast-like cells (EpiLC). As one example of such a modification, a novel O-GlcNAc modification was found in the transcription factor Sox2 at residue Ser263, and the modification site could be identified by nano liquid chromatography-mass spectrometry. | |||||
| 書誌情報 |
PLOS ONE (Online only:URL:http://www.plosone.org) 巻 17, 号 5, p. e0267804, 発行日 2022-05 |
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| 出版者 | ||||||
| 出版者 | PLOS | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0267804 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0267804 | |||||
