WEKO3
アイテム
Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach
https://repo.qst.go.jp/records/85671
https://repo.qst.go.jp/records/8567139521087-4a66-4cba-aeb0-22f676fb8716
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-03-07 | |||||
| タイトル | ||||||
| タイトル | Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yunoki, Yasuhiro
× Yunoki, Yasuhiro× Matsumoto, Atsushi× Morishima, Ken× Martel, Anne× Porcar, Lionel× Sato, Nobuhiro× Yogo, Rina× Tominaga, Taiki× Inoue, Rintaro× Maho, Yagi-Utsumi× Okuda, Aya× Shimizu, Masahiro× Urade, Reiko× Terauchi, Kazuki× Kono, Hidetoshi× Yagi, Hirokazu× Kato, Koichi× Sugiyama, Masaaki× Atsushi, Matsumoto× Hidetoshi, Kono |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the cyanobacterial circadian clock system, KaiA, KaiB and KaiC periodically assemble into a large complex. Here we determined the overall structure of their fully assembled complex by integrating experimental and computational approaches. Small-angle X-ray and inverse contrast matching small-angle neutron scatterings coupled with size-exclusion chromatography provided constraints to highlight the spatial arrangements of the N-terminal domains of KaiA, which were not resolved in the previous structural analyses. Computationally built 20 million structural models of the complex were screened out utilizing the constrains and then subjected to molecular dynamics simulations to examine their stabilities. The final model suggests that, despite large fluctuation of the KaiA N-terminal domains, their preferential positionings mask the hydrophobic surface of the KaiA C-terminal domain, hindering additional KaiA-KaiC interactions. Thus, our integrative approach provides a useful tool to resolve large complex structures harboring dynamically fluctuating domains. | |||||
| 書誌情報 |
Communications Biology 巻 5, p. 184, 発行日 2022-03 |
|||||
| 出版者 | ||||||
| 出版者 | Nature | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2399-3642 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s42003-022-03143-z | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s42003-022-03143-z | |||||
