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Formation of clustered DNA damage in vivo upon irradiation with ionizing radiation: Visualization and analysis with atomic force microscopy
https://repo.qst.go.jp/records/85602
https://repo.qst.go.jp/records/856021d17aed0-63ce-4f48-a24f-6f6af8fcca8f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-09-13 | |||||
| タイトル | ||||||
| タイトル | Formation of clustered DNA damage in vivo upon irradiation with ionizing radiation: Visualization and analysis with atomic force microscopy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Toshiaki, Nakano
× Toshiaki, Nakano× Ken, Akamatsu× Tsuda, Masataka× Tujimoto, Ayane× Ryoichi, Hirayama× Takeshi, Hiromoto× Taro, Tamada× Ide, Hiroshi× Naoya, Shikazono× Toshiaki, Nakano× Ken, Akamatsu× Ryoichi, Hirayama× Takeshi, Hiromoto× Taro, Tamada× Naoya, Shikazono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Clustered DNA damage is related to the biological effects of ionizing radiation. However, its precise yield and complexity (i.e., number of lesions per damaged site) in vivo remain unknown. To better understand the consequences of clustered DNA damage, a method was established to evaluate its yield and complexity in irradiated cells by atomic force microscopy. This was achieved by isolating and concentrating damaged DNA fragments from purified genomic DNA. It was found that X-rays and Fe ion beams caused clustered DNA damage in human TK6 cells, whereas Fenton's reagents did it less efficiently, highlighting clustered DNA damage as a signature of ionizing radiation. Moreover, Fe ion beams produced clustered DNA damage with high complexity. Remarkably, Fe ion beam–induced complex DNA double-strand breaks (DSBs) containing one or more base lesion(s) near the DSB end were refractory to repair, implying the lethal effect of complex DSBs. | |||||
| 書誌情報 |
Proceedings of the National Academy of Sciences of the United States of America 巻 119, 号 13, p. e2116132119, 発行日 2022-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-8424 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1073/pnas.2119132119 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.pnas.org/doi/10.1073/pnas.2119132119 | |||||
