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Mutation analysis of the DNA damage response regulator protein PprI in the radioresistant bacterium Deinococcus radiodurans
https://repo.qst.go.jp/records/85284
https://repo.qst.go.jp/records/85284e6ce51f8-3057-4e58-bd25-804834b01f9b
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-03-15 | |||||
| タイトル | ||||||
| タイトル | Mutation analysis of the DNA damage response regulator protein PprI in the radioresistant bacterium Deinococcus radiodurans | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Katsuya, Sato
× Katsuya, Sato× Sanzen, Toshihiko× Yutaka, Ono× Narumi, Issay× Katsuya, Sato× Yutaka, Ono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Deinococcus radiodurans is a representative strain of radioresistant bacteria and has extremely high resistance to various DNA damaging agents such as gamma rays, ultraviolet rays, desiccation, free radical-generating substances, and DNA cross-linkers. The previous studies revealed that the expression of a unique DNA repair-related protein, PprA, was up-regulated by a regulatory protein, PprI, following DNA damage in D. radiodurans. The radiation/desiccation response (RDR) motif was found in the upstream regions of the radiation-inducible genes (RDR regulons) by comparative genome sequence analysis of Deinococcus. The RDR motif serves as an operator sequence in the unique DNA repair response system. The PprI consists of three domains, namely metalloprotease, DNA binding and GAF-like binding domains. Another regulatory protein DdrO binds the RDR motif and serves as a repressor. Following DNA damage, the metalloprotease activity of PprI cleaves DdrO, resulting in derepression of the RDR regulon. In this study, to delineate the functional site of PprI in regard to the DNA damage response mechanism, we constructed several pprI-expression plasmids carrying mutations which cause a single amino acid substitution. The pprI-deleted mutant strains carrying the mutated pprI genes (PIM_E119Q and PIM_E149Q) showed significant sensitivity to gamma rays as same as the pprI-deletion strain. Moreover, unlike the strain DAW carrying the wild-type pprI gene, the enhances of luciferase reporter activity in the luciferase reporter strains carrying the mutated pprI genes (DAM_E119Q and DAM_E149Q) were not observed following irradiation. These results suggest that E119Q and E149Q at metalloprotease domain of PprI play a critical role in the DNA damage response mechanism. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2020 巻 QST-M-33, p. 85, 発行日 2022-03 |
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| 出版者 | ||||||
| 出版者 | QST | |||||
